Particle bombardment-mediated transformation of barley with an Arabidopsis NDPK2 cDNA

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Particle bombardment-mediated transformation of barley with an Arabidopsis NDPK2 cDNA
M O Um; T I Park; Y J Kim; H Y Seo; J G Kim; Suk Yoon KwonSang Soo Kwak; D J Yun; S J Yun
Bibliographic Citation
Plant Biotechnology Reports, vol. 1, no. 2, pp. 71-77
Publication Year
As barley is recalcitrant to transformation with current methods, a new improved system is required to apply genetic transformation in breeding programs. In a previous study, we defined optimal conditions for plant regeneration (PR) using mature embryos. This study was conducted to establish an improved transformation system employing the previously adjusted regeneration conditions. Optimal DNA delivery condition for the embryogenic calli developed from mature embryos was bombardment pressure of 1,100 psi at the target distance of 6 cm. The feasibility of the regeneration and DNA delivery conditions was confirmed by developing transgenic barley plants transformed with the Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) cDNA via particle bombardment of embryogenic calli from mature embryos. Stable integration of AtNDPK2 cDNA into barley genome was confirmed by PCR and Southern blot analysis of AtNDPK2 transgene. Transgenic plants showed about 10% reduction in membrane damage caused by methyl viologen, indicating the expression of AtNDPK2 transgene. The results demonstrated that the transformation system developed in this study employing the PR from mature embryo-derived embryonic callus is applicable in transgenic barley production.
barleynucleoside diphosphate kinase 2transformationmethyl viologenoxidative stress
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Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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