Phosphoproteomic analysis of AML14.3D10 cell line as a model system of eosinophilia = Eosinophilia 모델로서의 AML14.3D10 세포주의 인산화단백질체 연구

Cited 0 time in scopus
Metadata Downloads
Title
Phosphoproteomic analysis of AML14.3D10 cell line as a model system of eosinophilia = Eosinophilia 모델로서의 AML14.3D10 세포주의 인산화단백질체 연구
Author(s)
Soo In Ryu; Won Kon-KimHyun Ju Cho; Phil Young Lee; H Jung; Tae-Sung YoonJeong Hee MoonSunghyun KangHaryoung PooKwang-Hee BaeSang Chul Lee
Bibliographic Citation
BMB Reports, vol. 40, no. 5, pp. 765-772
Publication Year
2007
Abstract
Eosinophils act as effectors in the inflammatory reactions of allergic diseases including atopic dermatitis. Atopic dermatitis patients and others with allergic disorders suffer from eosinophilia, an accumulation of eosinophils due to increased survival or decreased apoptosis of eosinophils. In this study, a differential phosphoproteome analysis of AML14.3D10 eosinophil cell line after treatment with IL-5 or dexamethasone was conducted in an effort to identify the phosphoproteins involved in the proliferation or apoptosis of eosinophils. Proteins were separated by 2-DE and alterations in phosphoproteins were then detected by Pro-Q Diamond staining. The significant quantitative changes were shown in nineteen phosphoproteins including retinoblastoma binding protein 7, MTHSP75, and lymphocyte cytosolic protein 1. In addition, seven phosphoproteins including galactokinase I, and proapolipoprotein, were appeared after treatment with IL-5 or dexamethasone. Especially, the phospho-APOE protein was down-regulated in IL-5 treated AML14.3D10, while the more heavily phosphorylated APOE form was induced after dexamethasone treatment. These phosphoproteome data for the AML14.3D10 cell line may provide clues to understand the mechanism of eosinophilia as well as allergic disorders including atopic dermatitis.
Keyword
AML14.3D10atopic dermatitiseosinophiliaphosphoproteome
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5483/BMBRep.2007.40.5.765
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.