Identification and molecular modeling of a family 5 endocelluase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus

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Title
Identification and molecular modeling of a family 5 endocelluase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus
Author(s)
Dooil Kim; B H Park; B W Jung; M K Kim; S I Hong; Dae Sil Lee
Bibliographic Citation
International Journal of Molecular Sciences, vol. 7, no. 12, pp. 571-589
Publication Year
2007
Abstract
The genome of T. caldophilus GK24 was recently sequenced and annotated as 14 contigs, equivalent to 2.3 mega basepairs (Mbp) of DNA. In the current study, we identified a unique 13.7 kbp DNA sequence, which included the endocellulase gene of T. caldophilus GK24, which did not appear to be present in the complete genomic sequence of the closely related species T. thermophilus HB27 and HB8. Congo-red staining revealed a unique phenotype of cellulose degradation by strain GK24 that was distinct from other closely related Thermus strains. The results showed that strain GK24 is an aerobic, thermophilic, cellulolytic eubacterium which belongs to the group T. thermophilus. In order to understand the mechanism of production of cellobiose in T. caldophilus GK24, a three-dimensional model of the endocellulase, TcCel5A, was generated based on known crystal structures. Using this model, we carried out a flexible cellotetraose docking study.
Keyword
homology modelingmolecular dynamicscongo-red stainingglycosyl hydrolase family 5endocellulasethermus caldophilus GK24
ISSN
1422-0067
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/i7120571
Type
Article
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1. Journal Articles > Journal Articles
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