DNA methylation state is preserved in the sperm-derived pronucleus of the pig zygote

Cited 36 time in scopus
Metadata Downloads
Title
DNA methylation state is preserved in the sperm-derived pronucleus of the pig zygote
Author(s)
Y S Jeong; S Yeo; Jung Sun Park; Deog Bon Koo; W K Chang; Kyung Kwang Lee; Yong-Kook Kang
Bibliographic Citation
International Journal of Developmental Biology, vol. 51, no. 8, pp. 707-714
Publication Year
2007
Abstract
DNA methylation reprogramming (DMR) during preimplantation development erases differentiation-associated, unessential epigenetic information accumulated during gametogenesis, and ultimately brings pluripotency to the resulting embryo. Two patterns of DMR of sperm-derived pronucleus have been reported in mammals. In the first, the male pronucleus is actively demethylated whereas in the second, the methylation state seems to be maintained. The maintenance-type DMR has been seen only through immunocytochemical observations, and waits to be proven by additional molecular-level evidence. We demonstrate that, in pig, paternally derived DNA methylation is preserved during pronucleus development, based on the following observations. First, immunostaining of pig zygotes at different time points showed the DNA methylation state to be balanced between parental pronuclei throughout pronucleus development. Second, bisulfite analysis of PRE-1 repetitive sequences found mono- and polyspermic eggs to have similar methylation states. Third, the methylation state of a human erythropoietin gene delivered by transgenic pig spermatozoa was maintained in the male pronucleus. Finally, 5-aza-2′-deoxycytidine treatment, which blocks re-methylation, did not show the male pronucleus to be stalled in a demethylated state. In pig zygotes, paternally derived cytosine methylation was preserved throughout pronucleus development. These findings from multilateral DMR analyses provide further support to the view that DMR occurs in a non-conserved manner during early mammalian development.
Keyword
DemethylationDNA methylationMale pronucleusPig embryosReprogramming
ISSN
0214-6282
Publisher
Univ Basque Country Upv-Ehu Press
DOI
http://dx.doi.org/10.1387/ijdb.072450yj
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.