Analysis of intracellular short organic acid-coenzyme A esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry

Cited 53 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJ W Park-
dc.contributor.authorW S Jung-
dc.contributor.authorS R Park-
dc.contributor.authorByoung Chul Park-
dc.contributor.authorY J Yoon-
dc.date.accessioned2017-04-19T09:08:15Z-
dc.date.available2017-04-19T09:08:15Z-
dc.date.issued2007-
dc.identifier.issn10765174-
dc.identifier.uri10.1002/jms.1240ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8095-
dc.description.abstractA method employing silicone oil density centrifugation, solid-phase extraction (SPE) cleanup, and LC-ESI-MS/MS analysis was developed for the rapid, selective, sensitive, and quantitative detection of an intracellular pool of short organic acid-CoA esters in actinomycetes. The detection limit was determined to be approximately 0.8 pmol (1.2 ng/ml) for each standard CoA-ester analyzed by the present LC-ESI-MS/MS method. A selected ion chromatogram for a typical fragment ion (m/z 428) specific to CoA-esters enabled the detection of eight intracellular CoA-esters involved in both primary and secondary metabolisms. The application of this method to bacterial metabolomic study is demonstrated by the profiling of the intracellular CoA-ester pools in the wild-type Streptomyces venezuelae strain producing polyketide antibiotics (methymycin and pikromycin), a polyketide synthase (PKS)-deleted S. venezuelae mutant, and a S. venezuelae mutant expressing the heterologous PKS genes. By quantifying the individual CoA-esterlevel in three different genotypes of the S. Venezuela e strain, further insight could be gained into the role of CoA-estersin polyketide biosynthesis. This analytical approach can be extended to the quantification of the size and composition of in vivo CoA-ester pools in various microbes, and can provide a detailed understanding of the relationship between the in vivo CoA-ester pool and the production of pharmaceutically important polyketides.-
dc.publisherWiley-
dc.titleAnalysis of intracellular short organic acid-coenzyme A esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry-
dc.title.alternativeAnalysis of intracellular short organic acid-coenzyme A esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry-
dc.typeArticle-
dc.citation.titleJournal of Mass Spectrometry-
dc.citation.number9-
dc.citation.endPage1147-
dc.citation.startPage1136-
dc.citation.volume42-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.alternativeName박제원-
dc.contributor.alternativeName정원석-
dc.contributor.alternativeName박성렬-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName윤여준-
dc.identifier.bibliographicCitationJournal of Mass Spectrometry, vol. 42, no. 9, pp. 1136-1147-
dc.identifier.doi10.1002/jms.1240-
dc.subject.keywordActinomycetes-
dc.subject.keywordCoA-esters-
dc.subject.keywordLC-ESI-MS/MS-
dc.subject.keywordMetabolomics-
dc.subject.keywordPolyketide-
dc.subject.localActinomycetes-
dc.subject.localCoA-esters-
dc.subject.localLC-ESI-MS/MS-
dc.subject.localMetabolomics-
dc.subject.localPolyketide-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.