Establishment and maintenance of embryonic stem-like cell lines from In Vitro produced bovine blastocysts = 체외수정 유래 소 배반포로부터 유사 배아 줄기 세포의 확립 및 유지

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dc.contributor.authorYu Yeon Lee-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorJi Su Kim-
dc.contributor.authorBong Seok Song-
dc.contributor.authorYoun Joung Cho-
dc.contributor.authorJung Sun Park-
dc.contributor.authorDae Yeul Yu-
dc.contributor.authorD I Jin-
dc.contributor.authorKyung Kwang Lee-
dc.contributor.authorDeog Bon Koo-
dc.date.accessioned2017-04-19T09:08:15Z-
dc.date.available2017-04-19T09:08:15Z-
dc.date.issued2007-
dc.identifier.issnI0000006-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8097-
dc.description.abstractThis study was conducted to examine the establishment of bovine ES-like cells having pluripotency. The hatched blastocysts derived from culture of in vitro fertilized embryos for 10 to 12 days dissociated mechanically into ICM- and trophectoderm-rich clumps using needle, and cultured onto mitotically-inactivated MEF feeder layer. The primary colonies originated from ICM cells were detached mechanically 7 days after seeding and subsequent subculture was conducted at intervals of every 5 to 7 days. Two ES -like cell lines were established and maintained over 40 passages. Self-renewal of the established lines was confirmed by examining the alkaline phosphatase activity, stem cell-specific marker profiles including SSEA isotopes, Oct-4 and STAT3. Moreover, the established cell lines could produce anchorage-independent embryoid bodies (EBs) with gradual decrease of Oct-4 transcript level in time-dependent manner.-
dc.publisherSouth Korea-
dc.titleEstablishment and maintenance of embryonic stem-like cell lines from In Vitro produced bovine blastocysts = 체외수정 유래 소 배반포로부터 유사 배아 줄기 세포의 확립 및 유지-
dc.title.alternativeEstablishment and maintenance of embryonic ctem-like cell lines from In Vitro produced bovine blastocysts-
dc.typeArticle-
dc.citation.titleReproductive & Developmental Biology-
dc.citation.number3-
dc.citation.endPage220-
dc.citation.startPage215-
dc.citation.volume3-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorJi Su Kim-
dc.contributor.affiliatedAuthorBong Seok Song-
dc.contributor.affiliatedAuthorJung Sun Park-
dc.contributor.alternativeName이유연-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName김지수-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName조윤정-
dc.contributor.alternativeName박정선-
dc.contributor.alternativeName유대열-
dc.contributor.alternativeName진동일-
dc.contributor.alternativeName이경광-
dc.contributor.alternativeName구덕본-
dc.identifier.bibliographicCitationReproductive & Developmental Biology, vol. 3, no. 3, pp. 215-220-
dc.subject.keywordEmbryonic stem cell-
dc.subject.keywordWhole blastocyst-
dc.subject.keywordBovine-
dc.subject.keywordIn vitro-
dc.subject.localEmbryonic stem cell-
dc.subject.localWhole blastocyst-
dc.subject.localBovine-
dc.subject.localIn vitro-
dc.description.journalClassN-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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