Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media

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Title
Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media
Author(s)
T L M Stamford; T C M Stamford; N P Stamford; C E R S Santos; M C C P Lyra; Y H Park; Jin-Woo Bae; J M Araujo
Bibliographic Citation
World Journal of Microbiology & Biotechnology, vol. 23, no. 12, pp. 1719-1724
Publication Year
2007
Abstract
An endophytic actinomycete isolated from tubers of yam beam (Pachyrhizus erosus L. Urban) was classified as a novel species nominated Kitasatospora recifensis based in phenotypic and genotypic analysis (16S rDNA gene sequence). Monosporic culture using specific ISP2 media revealed three interspecies, which were identified by DNA southern hybridization (Wild strain 13817 W, Aerial Mycelium strain 13817 AM and Vegetative Mycelium strain 13817 VM). The strains were tested for the production of amylolitic enzymes in alternative media. Maximum yields for both enzymes were observed in starch-casein. Higher α-amylase was obtained with strain 13817 W in starch-urea, and amyloglucosidase with strain 13817 AM in starch-ammonium that are economic sources and may be important for industrial purposes. Type strain (DAUFPE 13817 T = KCTC 9972T= DSM 44943T).
Keyword
α-Amylase16S rDNAAmyloglucosidaseAmylolitic enzymesGene sequencePachyrhizus erosus
ISSN
0959-3993
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11274-007-9420-9
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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