Protein disulfide isomerase is cleaved by caspase-3 and -7 during apoptosis = 세포사멸 동안의 caspase-3과 -7에 의한 PDI의 절단

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dc.contributor.authorK S Na-
dc.contributor.authorByoung Chul Park-
dc.contributor.authorMi Jang-
dc.contributor.authorS Cho-
dc.contributor.authorDo Hee Lee-
dc.contributor.authorSunghyun Kang-
dc.contributor.authorC K Lee-
dc.contributor.authorKwang-Hee Bae-
dc.contributor.authorSung Goo Park-
dc.date.accessioned2017-04-19T09:08:17Z-
dc.date.available2017-04-19T09:08:17Z-
dc.date.issued2007-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8109-
dc.description.abstractApoptotic signals are typically accompanied by activation of aspartate-specific cysteine proteases called caspases, and caspase-3 and -7 play crucial roles in the execution of apoptosis. Previously, using the proteomic approach, protein disulfide isomerase (PDI) was found to be a candidate substrate of caspase-7. This abundant 55 kDa protein introduces disulfide bonds into proteins (via its oxidase activity) and catalyzes the rearrangement of incorrect disulfide bonds (via its isomerase activity). PDI is abundant in the ER but is also found in non-ER locations. In this study we demonstrated that PDI is cleaved by caspase-3 and -7 in vitro. In addition, in vivo experiment showed that it is cleaved during etoposide-induced apoptosis in HL-60 cells. Subcellular fractionation showed that PDI was also present in the cytosol. Furthermore, only cytosolic PDI was clearly digested by caspase-3 and -7. It was also confirmed by confocal image analysis that PDI and caspase-7 partially co-localize in both resting and apoptotic MCF-7 cells. Overexpression of cytosolic PDI (ER retention sequence deleted) inhibited cell death after an apoptotic stimulus. These data indicate that cytosolic PDI is a substrate of caspase-3 and -7, and that it has an anti-apoptotic action.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleProtein disulfide isomerase is cleaved by caspase-3 and -7 during apoptosis = 세포사멸 동안의 caspase-3과 -7에 의한 PDI의 절단-
dc.title.alternativeProtein disulfide isomerase is cleaved by caspase-3 and -7 during apoptosis-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number2-
dc.citation.endPage267-
dc.citation.startPage261-
dc.citation.volume24-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.affiliatedAuthorMi Jang-
dc.contributor.affiliatedAuthorDo Hee Lee-
dc.contributor.affiliatedAuthorSunghyun Kang-
dc.contributor.affiliatedAuthorKwang-Hee Bae-
dc.contributor.affiliatedAuthorSung Goo Park-
dc.contributor.alternativeName나경숙-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName장미-
dc.contributor.alternativeName조사연-
dc.contributor.alternativeName이도희-
dc.contributor.alternativeName강성현-
dc.contributor.alternativeName이종길-
dc.contributor.alternativeName배광희-
dc.contributor.alternativeName박성구-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 24, no. 2, pp. 261-267-
dc.subject.keywordapoptosis-
dc.subject.keywordcaspase-3-
dc.subject.keywordcaspase-7-
dc.subject.keywordprotein disulfide isomerase-
dc.subject.localApoptosis-
dc.subject.localapoptosis-
dc.subject.localCaspase 3-
dc.subject.localCaspase-3-
dc.subject.localcaspase-3-
dc.subject.localCaspase-7-
dc.subject.localcaspase-7-
dc.subject.localProtein disulfide isomerase (PDI)-
dc.subject.localprotein disulfide isomerase-
dc.subject.localprotein disulfide isomerase (PDI)-
dc.description.journalClassY-
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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