A quantitative analysis of N-myc downstream regulated gene 2 (NDRG 2) in human tissues and cell lysates by reverse-phase protein microarray

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dc.contributor.authorMi-Young Park-
dc.contributor.authorS C Choi-
dc.contributor.authorH S Lee-
dc.contributor.authorD K Kim-
dc.contributor.authorK E Baek-
dc.contributor.authorJong-Tae Kim-
dc.contributor.authorJ S Lim-
dc.contributor.authorYoung Il Yeom-
dc.contributor.authorJin Woong Chung-
dc.contributor.authorJ W Kim-
dc.contributor.authorP K Myung-
dc.contributor.authorHee Gu Lee-
dc.contributor.authorJae Wha Kim-
dc.contributor.authorEun Young Song-
dc.date.accessioned2017-04-19T09:08:21Z-
dc.date.available2017-04-19T09:08:21Z-
dc.date.issued2008-
dc.identifier.issn0009-8981-
dc.identifier.uri10.1016/j.cca.2007.09.010ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8133-
dc.description.abstractBackground: N-myc downstream regulated gene 2 (NDRG2) belongs to the NDRG family, which is comprised of 4 members, NDRG1-4. Recently, NDRG2 was reported as a new candidate for a tumor suppressor gene. We developed a reverse-phase protein microarray assay to access NDRG2 levels in human tissue specimens and cell lines. Methods: We synthesized recombinant NDRG2 protein and produced monoclonal antibodies (mAb) to the NDRG2 protein. We selected 2 hybridomas producing mAb that specifically recognize the NDRG2 protein. To determine the NDRG2 concentration, the samples of serially-diluted NDRG2 protein, cell lysate, or tissue lysate were spotted onto a nitrocellulose membrane-coated slide glass and allowed to react with the mAb to the NDRG2 protein. The reaction was followed by additional incubation with biotin-linked anti-mouse IgG and horseradish peroxidase (HRP)-conjugated streptavidin, subsequently. The addition of dimethylaminobenzidine induced color development, which was measured using the GenePix program. We determined the NDRG2 concentration in various tissue specimens and cell lines using the new protein microarray technique. Results: The dose-response relationship between NDRG2 and color intensity showed linearity in a range 0-10 ng/ml and a sensitivity of 50 pg/ml. The NDRG2 concentrations in the liver tissue lysates of patients with hepatocellular carcinoma (52.0 + 21.5 ng/mg) were significantly diminished as compared with those in the normal liver tissues (549.6 + 94.6 ng/mg). The results of the assay showed good agreement with those of Western blot analysis. Conclusions: The protein microarray is a highly sensitive and accurate method, and can adopted to assess specific proteins in human tissues or cell lines, particularly in the field of cancer and pathological research.-
dc.publisherElsevier-
dc.titleA quantitative analysis of N-myc downstream regulated gene 2 (NDRG 2) in human tissues and cell lysates by reverse-phase protein microarray-
dc.title.alternativeA quantitative analysis of N-myc downstream regulated gene 2 (NDRG 2) in human tissues and cell lysates by reverse-phase protein microarray-
dc.typeArticle-
dc.citation.titleClinica Chimica Acta-
dc.citation.number1-
dc.citation.endPage89-
dc.citation.startPage84-
dc.citation.volume387-
dc.contributor.affiliatedAuthorMi-Young Park-
dc.contributor.affiliatedAuthorJong-Tae Kim-
dc.contributor.affiliatedAuthorYoung Il Yeom-
dc.contributor.affiliatedAuthorJin Woong Chung-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.affiliatedAuthorJae Wha Kim-
dc.contributor.affiliatedAuthorEun Young Song-
dc.contributor.alternativeName박미영-
dc.contributor.alternativeName최승철-
dc.contributor.alternativeName이향신-
dc.contributor.alternativeName김대광-
dc.contributor.alternativeName백경은-
dc.contributor.alternativeName김종태-
dc.contributor.alternativeName임종석-
dc.contributor.alternativeName염영일-
dc.contributor.alternativeName정진웅-
dc.contributor.alternativeName김종완-
dc.contributor.alternativeName명평근-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeName김재화-
dc.contributor.alternativeName송은영-
dc.identifier.bibliographicCitationClinica Chimica Acta, vol. 387, no. 1, pp. 84-89-
dc.identifier.doi10.1016/j.cca.2007.09.010-
dc.subject.keywordhepatocellular carcinoma-
dc.subject.keywordmonoclonal antibody-
dc.subject.keywordN-myc downstream regulated gene 2-
dc.subject.keywordreverse-phase protein microarrary-
dc.subject.localHepatocellular carcinomas-
dc.subject.localHepatocellular carcinoma (HCC)-
dc.subject.localHepatocellular carcinoma-
dc.subject.localhepatocellular carcinoma (HCC)-
dc.subject.localhepatocellular carcinoma-
dc.subject.localMonoclonal antibody (mAb)-
dc.subject.localMonoclonal antibodies-
dc.subject.localMonoclonal Antibodies-
dc.subject.localMonoclonal antibody-
dc.subject.localmonoclonal antibody-
dc.subject.localMonoclonal Antibody-
dc.subject.localmonoclonal antibodies-
dc.subject.localN-myc downstream regulated gene 2-
dc.subject.localN-Myc downstream-regulated gene 2-
dc.subject.localreverse-phase protein microarrary-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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