Anti-tumor activity of ex vivo expanded cytokine-induced killer cells against human hepatocellular carcinoma

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Title
Anti-tumor activity of ex vivo expanded cytokine-induced killer cells against human hepatocellular carcinoma
Author(s)
Hwan Mook Kim; J Lim; Y D Yoon; J M Ahn; Jong Soon Kang; Kiho Lee; Song Kyu Park; Y J Jeong; J M Kim; G Han; K H Yang; Y J Kim; Y Kim; S B Han
Bibliographic Citation
International Immunopharmacology, vol. 7, no. 13, pp. 1793-1801
Publication Year
2007
Abstract
Cytokine-induced killer (CIK) cells are ex vivo expanded T cells with natural killer cell phenotypes and functions. In this study, the anti-tumor activity of CIK cells against hepatocellular carcinoma was evaluated in vitro and in vivo. In the presence of anti-CD3 antibody and IL-2 for 14 days, human peripheral blood mononuclear cell population changed to heterogeneous CIK cell population, which comprised 96% CD3+, 3% CD3¡?CD56+, 32% CD3+CD56+, 11% CD4+, 75% CD8+, and 30% CD8+CD56+. CIK cells produced significant amounts of IFN-γ and TNF-α; however, produced only slight amounts of IL-2, IL-4, and IL-5. At an effector-target cell ratio of 30:1, CIK cells destroyed 33% of SNU-354 human hepatocellular carcinoma cells, which was determined by the 51Cr-release assay. In addition, a dose of 1 × 106 CIK cells per mouse inhibited 60% of SNU-354 tumor growth in irradiated nude mice. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with hepatocellular carcinoma.
Keyword
Adoptive immunotherapyCytokine-induced killer cellsHepatocellular carcinoma
ISSN
1567-5769
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.intimp.2007.08.007
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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