Somatic embryogenesis and plant regeneration in tissue cultures of Artemisia annua L.

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Title
Somatic embryogenesis and plant regeneration in tissue cultures of Artemisia annua L.
Author(s)
P S Choi; Sung Ran Min; S M Ko; Jang Ryol Liu
Bibliographic Citation
Journal of Plant Biotechnology, vol. 34, no. 3, pp. 197-200
Publication Year
2007
Abstract
Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog`s (MS) medium supplemented with 4.52\;M 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.
ISSN
1229-2818
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2007.34.3.197
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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