Establishment of a simple and rapid method for quantitative determination of gamma-aminobutyric acid using 1H NMR and production of gamma-aminobutyric acid in cell suspension cultures of Coriandrum sativum L.

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dc.contributor.authorSuk Weon Kim-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T09:08:28Z-
dc.date.available2017-04-19T09:08:28Z-
dc.date.issued2007-
dc.identifier.issn1229-2818-
dc.identifier.uri10.5010/JPB.2007.34.3.207ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8160-
dc.description.abstractThis study describes a simple and rapid method for quantitative determination of \gamma-aminobutyric acid (GABA) using ^1H NMR spectroscopy from whole cell extracts of plant suspension cultures. When 9 cell lines derived from 8 species of higher plants maintained in liquid Marashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) were subjected to ^1H NMR, a cell line of Coriandrum sativum L. exhibited the highest level of GABA. The level reached up to 16.9 mg/dry wt when cells were cultured in MS medium supplemented with 0.5 mg/L 2,4-D after 3 weeks of incubation. The method for quantitative determination of GABA using ^1H NMR established in this study could be applied to high-throughput screening of various plant resources for GABA production and the cell suspension culture system of C. sativum could be further developed for commercial production of GABA.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEstablishment of a simple and rapid method for quantitative determination of gamma-aminobutyric acid using 1H NMR and production of gamma-aminobutyric acid in cell suspension cultures of Coriandrum sativum L.-
dc.title.alternativeEstablishment of a simple and rapid method for quantitative determination of gamma-aminobutyric acid using 1H NMR and production of gamma-aminobutyric acid in cell suspension cultures of Coriandrum sativum L.-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number3-
dc.citation.endPage212-
dc.citation.startPage207-
dc.citation.volume34-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.alternativeName김석원-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 34, no. 3, pp. 207-212-
dc.identifier.doi10.5010/JPB.2007.34.3.207-
dc.description.journalClassN-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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