Establishment of a simple and rapid method for quantitative determination of gamma-aminobutyric acid using 1H NMR and production of gamma-aminobutyric acid in cell suspension cultures of Coriandrum sativum L.

Abstract

This study describes a simple and rapid method for quantitative determination of \gamma-aminobutyric acid (GABA) using ^1H NMR spectroscopy from whole cell extracts of plant suspension cultures. When 9 cell lines derived from 8 species of higher plants maintained in liquid Marashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) were subjected to ^1H NMR, a cell line of Coriandrum sativum L. exhibited the highest level of GABA. The level reached up to 16.9 mg/dry wt when cells were cultured in MS medium supplemented with 0.5 mg/L 2,4-D after 3 weeks of incubation. The method for quantitative determination of GABA using ^1H NMR established in this study could be applied to high-throughput screening of various plant resources for GABA production and the cell suspension culture system of C. sativum could be further developed for commercial production of GABA.