Plant regeneration from leaf and root cultures of Lycoris chejuensis via bulblet formation = 제주상사화(Lycoris chejuensis K. tae et S. Ko) 잎 및 뿌리 절편으로부터 소자구 형성을 통한 식물체 재생안
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- Plant regeneration from leaf and root cultures of Lycoris chejuensis via bulblet formation = 제주상사화(Lycoris chejuensis K. tae et S. Ko) 잎 및 뿌리 절편으로부터 소자구 형성을 통한 식물체 재생안
- Myung Jin Oh; Jong Mi Park; K H Tae; Jang Ryol Liu; Suk Weon Kim
- Bibliographic Citation
- Journal of Plant Biotechnology, vol. 34, no. 3, pp. 223-227
- Publication Year
- Plant regeneration system from leaf and root segments of Lycoris chejuensis via bulblet formation was established. Surface-sterilized leaf and root segments were cultured on the B5 medium containing 2,4-D. After 12 weeks of culture onto B5 medium containing 2,4-D, white globular structures and white calluses were formed on the cut surface of the explants. The highest frequency of globular structures and calluses formation from leaf explants was 32.1% when leaf explants were cultured onto B5 medium supplemented with 1 mg/L of 2,4-D. However, the higher concentration of 2,4-D (over than 3 mg/L) resulted in decrease of the frequency. In comparison to leaf explants, root segments showed the highest frequency at a rate of 36.1% when root explants were cultured onto B5 medium supplemented with 3 mg/L of 2,4-D. These structures and calluses were sub-cultured and proliferated onto the same culture medium. Upon transfer to B5 basal medium, white globular structures were developed into bulblets and normal plantlets. After 4 weeks of incubation in the light, plantlets were successfully rooted over the frequency of approximately 90%. Rooted plantlets were successfully transferred to potting soil and acclimatized in the growth chamber. The plant regeneration system of Lycoris chejuensis established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.
- South Korea
- Appears in Collections:
- Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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