Enhancement of chloroplast transformation frequency by using mesophyll cells containing a few enlarged chloroplasts from nuclear transformed plants in tobacco

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dc.contributor.authorWon Joong Chung-
dc.contributor.authorSung Ran Min-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T09:08:28Z-
dc.date.available2017-04-19T09:08:28Z-
dc.date.issued2007-
dc.identifier.issn12292818-
dc.identifier.uri10.5010/JPB.2007.34.3.271ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8163-
dc.description.abstractn the chloroplast transformation process, a chloroplast containing transformed chloroplast genome copies should be selected over wild-type chloroplasts on selection medium. It is more effective for a cell to become homoplasmic if the cell contains smaller number of chloroplasts. Therefore, to reduce the number of chloroplasts in mesophyll cells in tobacco, we overexpressed FtsZ to generate transgenic plants, of which mesophyll cell contained a few enlarged chloroplasts contrast to a wild-type mesophyll cell containing approximately 100 chloroplasts. It was demonstrated that transgenic leaf tissues comprising cells with a few enlarged chloroplasts gave rise to approximately 40% higher frequency of chloroplast-transformed adventitious shoots.-
dc.publisherSouth Korea-
dc.titleEnhancement of chloroplast transformation frequency by using mesophyll cells containing a few enlarged chloroplasts from nuclear transformed plants in tobacco-
dc.title.alternativeEnhancement of chloroplast transformation frequency by using mesophyll cells containing a few enlarged chloroplasts from nuclear transformed plants in tobacco-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number3-
dc.citation.endPage275-
dc.citation.startPage271-
dc.citation.volume34-
dc.contributor.affiliatedAuthorWon Joong Chung-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.alternativeName정원중-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 34, no. 3, pp. 271-275-
dc.identifier.doi10.5010/JPB.2007.34.3.271-
dc.description.journalClassN-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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