Development of a simple and rapid immunochromatographic strip test for diarrhea-causative porcine rotavirus in swine stool

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dc.contributor.authorJ H Kang-
dc.contributor.authorDur Han Kwon-
dc.contributor.authorT W Chung-
dc.contributor.authorY D Kim-
dc.contributor.authorHee Gu Lee-
dc.contributor.authorJae Wha Kim-
dc.contributor.authorIn Seong Choe-
dc.contributor.authorK W Kim-
dc.contributor.authorJ S Lim-
dc.contributor.authorEun Young Song-
dc.contributor.authorC H Kim-
dc.date.accessioned2017-04-19T09:08:28Z-
dc.date.available2017-04-19T09:08:28Z-
dc.date.issued2007-
dc.identifier.issn0166-0934-
dc.identifier.uri10.1016/j.jviromet.2007.06.002ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8164-
dc.description.abstractA rapid and simple immunochromatography (IC) strip test, for specific detection of porcine rotavirus (PRV) in stool specimen, was developed. Monoclonal antibodies (mAbs) to the OSU strain of PRV have been produced in mice. Among them, two hybridoma clones that generate mAb-1 and mAb-2, respectively, specific for VP6 protein of PRV, have been selected. In the IC configuration, mAb-1, one of the selected mAbs was used to the designed coat microparticles (MP), while another mAb-2 was used to fix it on the nitrocellulose membrane strip to form a result line. The control line was formed on the same membrane strip past the result line by fixing anti-mouse IgG antibody. The IC test was capable of detecting 1000 plaque-forming units of PRV/ml in less than 5 min, and the binding capacity was demonstrated by specific recognition of PRV only, but not other porcine diarrhea viruses, transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV). The IC test produced positive results with all the nine PRV-positive stool specimens and negative results with five different non-PRV specimens, which were identified previously by the polymerase chain reaction (PCR) test, respectively. The results indicate an excellent concordance between the two methods, suggesting a potential application of the three combinated IC tests (PRV, TGEV and PEDV) for the on-site, rapid screening of porcine diarrhea cases.-
dc.publisherElsevier-
dc.titleDevelopment of a simple and rapid immunochromatographic strip test for diarrhea-causative porcine rotavirus in swine stool-
dc.title.alternativeDevelopment of a simple and rapid immunochromatographic strip test for diarrhea-causative porcine rotavirus in swine stool-
dc.typeArticle-
dc.citation.titleJournal of Virological Methods-
dc.citation.number1-
dc.citation.endPage79-
dc.citation.startPage74-
dc.citation.volume146-
dc.contributor.affiliatedAuthorDur Han Kwon-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.affiliatedAuthorJae Wha Kim-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.affiliatedAuthorEun Young Song-
dc.contributor.alternativeName-
dc.contributor.alternativeName권두한-
dc.contributor.alternativeName정태화-
dc.contributor.alternativeName김영대-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeName김재화-
dc.contributor.alternativeName최인성-
dc.contributor.alternativeName-
dc.contributor.alternativeName임종석-
dc.contributor.alternativeName송은영-
dc.contributor.alternativeName김철호-
dc.identifier.bibliographicCitationJournal of Virological Methods, vol. 146, no. 1, pp. 74-79-
dc.identifier.doi10.1016/j.jviromet.2007.06.002-
dc.subject.keywordImmunochromatography strip-
dc.subject.keywordMonoclonal antibody-
dc.subject.keywordPorcine diarrhea-
dc.subject.keywordPorcine rotavirus-
dc.subject.keywordVP6 protein-
dc.subject.localImmunochromatography strip-
dc.subject.localMonoclonal antibody (mAb)-
dc.subject.localMonoclonal antibodies-
dc.subject.localMonoclonal Antibodies-
dc.subject.localMonoclonal antibody-
dc.subject.localmonoclonal antibody-
dc.subject.localMonoclonal Antibody-
dc.subject.localmonoclonal antibodies-
dc.subject.localporcine diarrhea-
dc.subject.localPorcine diarrhea-
dc.subject.localPorcine rotavirus-
dc.subject.localVP6 protein-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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