Cardiac cell differentiation of mouse embryonic stem cells in the co-culture with fetal heart

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Title
Cardiac cell differentiation of mouse embryonic stem cells in the co-culture with fetal heart
Author(s)
Koung Min Park; Eun Kyoung KimHae Rim Kim; Og Sung Moon; Young Suk WonHyoung-Chin Kim; B Ahn; S H Han; Ki Hoan Nam
Bibliographic Citation
Laboratory Animal Research, vol. 23, no. 3, pp. 321-326
Publication Year
2007
Abstract
To investigate the effect of fetal cardiac cells on the differentiation of embryonic stem cells (ESCs) into cardiomyocytes, Mouse ESCs were co-cultured with heart cells prepared from fetus aged 11.5, 12.5, 13.5 or 14.5 days postcoitum (dpc). The fetal mouse heart was from ICR and 129 strains from which KH2 ES cell line was derived. In the present study, KH2 cells expressing EGFP directed by α-myosin heavy chain gene promoter were co-cultured with fetal heart isolated at different fetal stages. The cardiomyocytes derived from ESC in the co-culture could be identified by their EGFP expression. The EGFP expressing cells which were confirmed to be cardiomyocytes were measured as an indicator of cardiac cell differentiation of ESC. The fetal cardiac cells from 12.5 dpc were more effective in cardiac cell differentiation of ESC than the cells from fetus aged 11.5, 13.5 or 14.5 dpc. Fetal heart from 129 strain which is immunologically compatible with KH2 cells showed more strong cardiac cell differentiation induction activity compared with that from ICR mice. As the results, we demonstrated that fetal hearts at different developmental stages have different cardiac cell differentiation induction activity from ESC implying that fetal heart expresses cardiac cell differentiation induction factor(s).
Keyword
embryonic stem cellscardiac cell differentiationfetal heartco-culture
ISSN
1738-6055
Publisher
Springer-BMC
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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