Shewanella haliotis sp. nov., isolated from the gut microflora of abalone, Haliotis discus hannai

Abstract

A motile, rod-shaped, pink-orange pigmented bacterium, designated strain DW01T, was isolated from the gut microflora of abalone collected from the South Sea (Republic of Korea). Cells were Gram-negative, facultatively anaerobic, catalase- and oxidase-positive. The major fatty acids were iso-C15:0 (17.7 %), C16:0 (13.4 %), iso-C15:0 2-OH and/or C16:1ω7c (12.5 %) and C17:1ω8C (10.7 %). The DNA G + C content was 53.7 mol%. A phylogenetic tree based on the 16S rRNA gene sequences showed that strain DW01T forms a lineage of the genus Shewanella and is closely related to Shewanella algae ATCC 51192T (98.3 % sequence similarity) and to other members of the genus Shewanella (91.0-94.9%). The phenotypic characteristics and DNA-DNA hybridization relatedness data indicate that strain DW01T should be distinguished from S. algae ATCC 51192T. On the basis of the data presented in this study, strain DW01T represents a novel species, for which the name Shewanella haliotis sp. nov. is proposed. The type strain is DW01T (=KCTC 12896T=JCM 14758T) ？