Cohnella laeviribosi sp. nov., isolated from a volcanic pond

Abstract

A novel thermophilic and endospore-forming Gram-positve bacterium capable of assimilating and isomerizing L-ribose was isolated from a volcanic area in Likupang, Indonesia. The isolate, RI-39T, was able to grow at high temperatures (37-60 °C); optimum growth was observed at pH 6.5 and 45 °C. The strain contained MK-7 (87 %) as the main respiratory quinone and had a DNA G + C content of 51 mol%. The major cellular fatty acids of the isolate were iso-C16:0 and anteiso-C15:0 and the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents an evolutionary lineage that is distinct from those of other Cohnella species. Based on morphological, physiological and chemotaxonomic characteristics and 16S rRNA gene sequence comparisons, it is proposed that strain RI-39T represents a novel species, Cohnella laeviribosi sp. nov. The type strain is RI-39T (=KCTC 3987T =KCCM 10653PT =CCUG 552217T).