Generation and characterization of a monoclonal antibody with specificity for Mycoplasma arginini

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Title
Generation and characterization of a monoclonal antibody with specificity for Mycoplasma arginini
Author(s)
Yeon Sung Son; Hyo Jeong Hong
Bibliographic Citation
Journal of Microbiology, vol. 45, no. 6, pp. 547-552
Publication Year
2007
Abstract
Previously, we generated monoclonal antibodies (MAbs) that bound to the surface of human embryonic stem cells (hESCs) in an attempt to discover new hESC-specific surface markers. In this study, MAb 47-235 (IgG1, κ) was selected for further characterization. The MAb bound to the surface of undifferentiated hESCs but did not bind to mouse ESCs or mouse embryonic fibroblast cells in flow cytometric analysis. The antibody immunoprecipitated a 47 kDa protein from the lysates of cell surface-biotinylated hESCs. Identification of the protein by quadrupole time of flight tandem mass spectrometry revealed that 47-235 binds to Ag 243-5 protein of Mycoplasma arginini. BM-Cyclin treatment of the hESCs that reacted with 47-235 resulted in loss of mycoplasma DNA and the reactivity to 47-235. Nevertheless, the hESCs that were reactive to 47-235 maintained self-renewal and pluripotency and thus could be differentiated into three embryonic germ layers.
Keyword
cell surface proteinhuman embryonic stem cellsmonoclonal antibodymycoplasma arginini
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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