Inhibition of starch biosynthesis by antisense expression of cDNAs encoding ADP-glucose pyrophosphorylase small subunit in sweet potato = 고구마에서 ADP-glucose pyrophosphorylase small subunit cDNA의 Antisense 발현에 의한 전분생합성 저해

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Title
Inhibition of starch biosynthesis by antisense expression of cDNAs encoding ADP-glucose pyrophosphorylase small subunit in sweet potato = 고구마에서 ADP-glucose pyrophosphorylase small subunit cDNA의 Antisense 발현에 의한 전분생합성 저해
Author(s)
Sung Ran Min; J M Bae; C H Harn; Won Joong Chung; Y B Lee; Jang Ryol Liu
Bibliographic Citation
Journal of Plant Biotechnology, vol. 34, no. 4, pp. 277-283
Publication Year
2007
Abstract
Embryogenic calluses derived from shoot apical meristem explants of sweet potato were subjected to particle bombardment to generate transgenic plants for antisense expression of cDNAs encoding two different AGPase small subunit (ibAGP1 and ibAGP2). Plants were generated via somatic embryogenesis. PCR and Southern analysis demonstrated that the incorporation of ibAGP1 and ibAGP2 into the genome in an antisense orientation. Immunoblot analysis confirmed reduced levels of AGPase small subunit in transgenic plant leaves. Plants with both ibAGP1 and ibAGP2 produced a lower level of the protein than plants with ibAGP1 alone. Iodine test demonstrated that transgenic plant leaves and storage root accumulated reduced amounts of starch. Iodine staining of leaf tissues indicated that transgenic plants accumulated less amount of starch than control. In accordance with western blot analysis, plants with both ibAGP1 and ibAGP2 accumulated a lower amount of starch than plants with ibAGP1 alone. Both transgenic plants exhibited a severely retarded growth, resulting in bare survival. It is suggested that disrupted expression of the gene encoding AGPase small subunit is lethal to the growth of sweet potato contrast to other species including potato.
ISSN
1229-2818
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2007.34.4.277
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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