Mass production of gain-of function mutants of hair roots in ginseng = 기능획득 돌연변이 인삼 모상근의 대량생산

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Mass production of gain-of function mutants of hair roots in ginseng = 기능획득 돌연변이 인삼 모상근의 대량생산
S M Ko; D S In; H J Chung; D W Choi; Jang Ryol Liu
Bibliographic Citation
Journal of Plant Biotechnology, vol. 34, no. 4, pp. 285-291
Publication Year
This study describes conditions for the mass production of activation-tagged mutant hairy root lines of ginseng by cocultivation with Agrobacterium rhizogenes. Because it is not currently possible to produce progeny from transgenic ginseng, a loss-of-function approach for functional genomics cannot be appliable to this species. A gain-of-function approach is alternatively the choice and hairy root production by cocultivation of A. rhizogenes would be most practical to obtain a large number of mutants. Various sources of explants were subjected to genetic transformation with various strains of A. rhizogenes harboring the activation-tagging vector pKH01 to determine optimum conditions for the highest frequency of hairy root formation on explants. Petiole explants cocultivated with A. rhizogenes R1000 produced hairy roots at a frequency of 85.9% after 4 weeks of culture. Conditions for maximum growth or branching rate of hairy roots were also investigated by using various culture media. Petiole explants cultured on half strength Schenk and Hildebrandt medium produced vigorously growing branched roots at a rate of 2.6 after 4 weeks of culture. A total of 1,989 lines of hairy root mutants were established in this study. These hairy root lines will be useful to determine functions of genes for biosynthesis of ginsenosides.
Korea Soc-Assoc-Inst
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