Induction of axenic culture of Arthrospira (Spirulina) platensis based on antibiotic sensitivity of contaminating bacteria

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Title
Induction of axenic culture of Arthrospira (Spirulina) platensis based on antibiotic sensitivity of contaminating bacteria
Author(s)
Gang Guk Choi; M S Bae; Chi-Yong AhnHee-Mock Oh
Bibliographic Citation
Biotechnology Letters, vol. 30, no. 1, pp. 87-92
Publication Year
2008
Abstract
Arthrospira platensis SAG 21.99 and the isolated bacteria (Halomonas spp., Staphylococcus sp., etc.) from the culture of A. platensis SAG 21.99 were treated with five antibiotics to determine the minimal lethal concentrations. The combination of a washing step and a consecutive treatment with antibiotics, imipenem (100 μg ml-1), neomycin (100 μg ml-1) and cycloheximide (20 μg ml-1), treatment step was highly effective in eliminating bacteria. An axenic culture of A. platensis SAG 21.99 could be induced within 3 days using this method. This technique is a simple and rapid method for obtaining axenic cultures of filamentous cyanobacteria.
Keyword
Arthrospira platensisAxenic cultureCyanobacteriaImipenemNeomycinAntibiotics
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-007-9523-2
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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