Cloning, expression, purification, crystallization and X-ray crystallographic analysis of ScpB (Rv1710) from Mycobacterium tuberculosis

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dc.contributor.authorS Y Kwon-
dc.contributor.authorB S Kang-
dc.contributor.authorMyung Hee Kim-
dc.contributor.authorK J Kim-
dc.date.accessioned2017-04-19T09:08:45Z-
dc.date.available2017-04-19T09:08:45Z-
dc.date.issued2007-
dc.identifier.citationActa Crystallographica. Section F, Structural Biology and Crystallization Communications,63,12,1058,1060ko
dc.identifier.issn1744-3091-
dc.identifier.uri10.1107/S1744309107056953ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8221-
dc.description.abstractStructural maintenance of chromosome (SMC) proteins play diverse roles in cellular DNA reassembly by directly interacting with DNA. They require non-SMC proteins for their proper function; these include the conserved segregation and condensation proteins (Scps) in prokaryotes. ScpB from Mycobacterium tuberculosis was crystallized using the sitting-drop vapour-diffusion method in the presence of 2 M NaCl and 10% PEG 6000 at 295 K. X-ray diffraction data were collected to a maximum resolution of 2.3 ? at a synchrotron beamline. The crystal belongs to the hexagonal space group R32, with unit-cell parameters a = b = 136.69, c = 78.55 ?, γ = 120°. With one molecule per asymmetric unit, the crystal volume per unit protein weight (V M) is 2.95 ?3 Da-1. The structure was solved by the single anomalous dispersion method and structure refinement is in progress.-
dc.publisherInt Union Crystallography-
dc.titleCloning, expression, purification, crystallization and X-ray crystallographic analysis of ScpB (Rv1710) from Mycobacterium tuberculosis-
dc.title.alternativeCloning, expression, purification, crystallization and X-ray crystallographic analysis of ScpB (Rv1710) from Mycobacterium tuberculosis-
dc.typeArticle-
dc.citation.titleActa Crystallographica Section F-Structural Biology-
dc.citation.number12-
dc.citation.endPage1060-
dc.citation.startPage1058-
dc.citation.volume63-
dc.contributor.affiliatedAuthorMyung Hee Kim-
dc.contributor.alternativeName권수영-
dc.contributor.alternativeName강범식-
dc.contributor.alternativeName김명희-
dc.contributor.alternativeName김경진-
dc.identifier.bibliographicCitationActa Crystallographica Section F-Structural Biology, vol. 63, no. 12, pp. 1058-1060-
dc.identifier.doi10.1107/S1744309107056953-
dc.subject.keywordScpB-
dc.subject.keywordSegregation and condensation proteins-
dc.subject.localScpB-
dc.subject.localSegregation and condensation proteins-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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