Metabolic discrimination of sucrose starvation from Arabidopsis cell suspension by 1H NMR based metabolomics

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Title
Metabolic discrimination of sucrose starvation from Arabidopsis cell suspension by 1H NMR based metabolomics
Author(s)
Suk Weon Kim; B C Ku; Jong Hyun Kim; Jang Ryol Liu
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 12, no. 6, pp. 653-661
Publication Year
2007
Abstract
Whole cell extracts of Arabidopsis cell cultures maintained on various sucrose concentrations (0, 3, and 6%) were analyzed by 1H NMR spectroscopy to determine the comprehensive metabolic change in these cultures during sucrose starvation. The amount of sucrose, glucose, and fructose in the cells decreased to almost nothing after 12 h of culture in medium without sucrose. In contrast, the total free amino acid content of the cells increased as the culture proceeded. Among the free amino acids, phenylalanine and malic acid increased the most, followed by asparagine and alanine, whereas glutamic acid did not change significantly. These results are in agreement with previous studies using HPLC. 1H NMR spectroscopy enabled measurement of changes in the sugar and free amino acid content of whole cell extracts without fractionation and complicated sample preparation. These results indicate that comprehensive metabolic changes in the cells can be determined by a simple, rapid method using whole cell extracts and 1H NMR spectroscopy.
Keyword
1H NMR spectroscopyArabidopsis thaliana cell suspensionPrincipal component analysisPrincipal component regressionSucrose starvation
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF02931082
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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