Localization of 5,105 hanwoo (Korean cattle) BAC clones on bovine chromosomes by the analysis of BAC end sequences (BESS) involving 21,024 clones

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Title
Localization of 5,105 hanwoo (Korean cattle) BAC clones on bovine chromosomes by the analysis of BAC end sequences (BESS) involving 21,024 clones
Author(s)
J M Choi; S H Chae; S W Kang; D S Choi; Y S Lee; Hong-Seog Park; J S Yeo; I Choi
Bibliographic Citation
Asian-Australasian Journal of Animal Sciences, vol. 20, no. 11, pp. 1636-1650
Publication Year
2007
Abstract
As an initial step toward a better understanding of the genome structure of Korean cattle (Hanwoo breed) and initiation of the framework for genomic research in this bovine, the bacterial artificial chromosome (BAC) end sequencing of 21,024 clones was recently completed. Among these clones, BAC End Sequences (BESs) of 20,158 clones with high quality sequences (Phred score >20. average BES equaled 620 bp and totaled 23,585,814 bp), after editing sequencing results by eliminating vector sequences, were used initially to compare sequence homology with the known bovine chromosomal DNA sequence by using BLASTN analysis. Blast analysis of the BESs against the NCBI Genome database for Bos taurus (Build 2.1) indicated that the BESs from 13.201 clones matched bovine contig sequences with significant blast hits (E< accuracy. high very with chromosome bovine the to mapped were analysis, secondary from clones 1.052 and analysis primary 4.053 including hits, paired-end BAC cattle Korean of 5.105 total a Finally, hits. 6.126 hits single-end 7,075 40).>
Keyword
BAC end sequence (BES)Chromosomal mapKorean cattle (Hanwoo)
ISSN
1011-2367
Publisher
Asian-Australasian Assoc Animal Production Soc
DOI
http://dx.doi.org/10.5713/ajas.2007.1636
Type
Article
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1. Journal Articles > Journal Articles
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