Proteomic analysis of pancreas derived from adult cloned pig

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Title
Proteomic analysis of pancreas derived from adult cloned pig
Author(s)
Jung Il Chae; Young Keun Cho; S K Cho; J H Kim; Y M Han; Deog Bon Koo; Kyung Kwang Lee
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 366, no. 2, pp. 379-387
Publication Year
2008
Abstract
The potential medical applications of animal cloning include xenotransplantation, but the complex molecular cascades that control porcine organ development are not fully understood. Still, it has become apparent that organs derived from cloned pigs may be suitable for transplantation into humans. In this study, we examined the pancreas of an adult cloned pig developed through somatic cell nuclear transfer (SCNT) using two-dimensional electrophoresis (2-DE) and Western blotting. Proteomic analysis revealed 69 differentially regulated proteins, including such apoptosis-related species as annexins, lamins, and heat shock proteins, which were unanimously upregulated in the SCNT sample. Among the downregulated proteins in SCNT pancreas were peroxiredoxins and catalase. Western blot results indicate that several antioxidant enzymes and the anti-apoptotic protein were downregulated in SCNT pancreas, whereas several caspases were upregulated. Together, these data suggest that the accumulation of reactive oxygen species (ROS) in the pancreas of an adult cloned pig leads to apoptosis.
Keyword
apoptosispancreaspigproteomic analysisROSsomatic cell cloning
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2007.11.114
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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