Variations in protein glycosylation in Hansenula polymorpha depending on cell culture stage

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Title
Variations in protein glycosylation in Hansenula polymorpha depending on cell culture stage
Author(s)
S Y Kim; Jung Hoon Sohn; Y R Pyun; Eui Sung Choi
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 17, no. 12, pp. 1949-1954
Publication Year
2007
Abstract
A simple way to prevent protein hyperglycosylation in Hansenula polymorpha was found. When glucose oxidase from Aspergillus niger and carboxymethyl cellulase from Bacillus subtilis were expressed under the control of an inducible methanol oxidase (MOX) promoter using methanol as a carbon source, hyperglycosylated forms occurred. In contrast, MOX-repressing carbon sources (e.g., glucose, sorbitol, and glycerol) greatly reduced the extent of hyperglycosylation. Carbon source starvation of the cells also reduced the level of glycosylation, which was reversed to hyperglycosylation by the resumption of cell growth. It was concluded that the proteins expressed under actively growing conditions are produced as hyperglycosylated forms, whereas those under slow or nongrowing conditions are as short-glycosylated forms. The prevention of hyperglycosylation in the Hansenula polymorpha expression system constitutes an additional advantage over the traditional Saccharomyces cerevisiae system in recombinant production of glycosylated proteins.
Keyword
CMCaseglucose oxidaseglycosylationhansenula polymorpha
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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