Effect of 5-O-methylhirsutanonol on nuclear factor-κB-dependent production of NO and expression of iNOS in lipopolysaccharide-Induced RAW264.7 cells = LPS처리에 의해 유도된 NF-kB에 의존하는 iNOS

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Title
Effect of 5-O-methylhirsutanonol on nuclear factor-κB-dependent production of NO and expression of iNOS in lipopolysaccharide-Induced RAW264.7 cells = LPS처리에 의해 유도된 NF-kB에 의존하는 iNOS
Author(s)
Jong Min Han; Woo Song Lee; J R Kim; J Son; Oh Hyung Kwon; Hyun Jun Lee; Jung Joon Lee; Tae Sook Jeong
Bibliographic Citation
Journal of Agricultural and Food Chemistry, vol. 56, no. 1, pp. 92-98
Publication Year
2008
Abstract
Diarylheptanoids are known to have anti-inflammatory and anti-atherosclerotic activities in various cell types, including macrophages. 5-O-Methylhirsutanonol (5-MH) isolated from the leaves of Alnus japonica Steud exhibited the antioxidant activities on Cu2+- and AAPH-mediated low-density lipoprotein (LDL) oxidation in the thiobarbituric acid-reactive substances (TBARS) assay as well as the macrophage-mediated LDL oxidation. In the main study, we examined anti-inflammatory activities of 5-O- methylhirsutanonol (5-MH) on nuclear factor κB (NF-κB)-dependent nitric oxide (NO) production and expression of inducible nitric oxide synthease (iNOS) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. 5-MH inhibited NO production with an IC50 value of 14.5 μM and expression of both iNOS protein and iNOS mRNA in a parallel dose-response manner. Then, expression of inflammation-associated genes, such as TNF-α, COX-2, and IL-1β, was suppressed by 5-MH, as determined by reverse transcriptase polymerase chain reaction analysis. Moreover, 5-MH attenuated NF-κB activation by inhibition of hyperphosphorylation of IκB-α and its subsequent proteolytic degradation and p65 nuclear translocation, as well as preventing DNA-binding ability. In addition, 5-MH suppressed the mRNA expression of the gene reactive oxygen species (ROS) concerned in the regulation of NF-κB signaling.
Keyword
5-O-methylhirsutanonol (5-MH)DiarylheptanoidsInducible nitric oxide synthease (iNOS)Nitric oxide (NO)Nuclear factor κB (NF-κB)Reactive oxygen species (ROS)
ISSN
0021-8561
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/jf0721085
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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