Effect of safflower seeds supplementation on stimulation of the proliferation, differentiation and mineralization of osteoblastic MC3T3-E1 cells

Cited 26 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorK W Kim-
dc.contributor.authorS J Suh-
dc.contributor.authorT K Lee-
dc.contributor.authorK T Ha-
dc.contributor.authorJ K Kim-
dc.contributor.authorK H Kim-
dc.contributor.authorD I Kim-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorT C Moon-
dc.contributor.authorC H Kim-
dc.date.accessioned2017-04-19T09:08:49Z-
dc.date.available2017-04-19T09:08:49Z-
dc.date.issued2008-
dc.identifier.issn0378-8741-
dc.identifier.uri10.1016/j.jep.2007.09.003ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8240-
dc.description.abstractAnti-bone resorption properties of the Korean herbal formulation, Gami-Honghwain (HJ), which comprises Carthamus tinctorius L. seed and hominis placenta, were investigated. We demonstrate that the production of PGE2 is inhibited by 20-100 μg/ml HJ in nontransformed osteoblastic cells (MC3T3-E1 cells), indicating that HJ inhibits PGE2 production. The effect of HJ on the proliferation and osteoblastic differentiation in MC3T3-E1 was also studied. HJ dose-dependently increased DNA synthesis (significant at 20-100 μg/ml), and increased alkaline phosphatase (ALP) and prolyl hydroxylase activities of MC3T3-E1 cells (20-100 μg/ml), while anti-estrogen tamoxifen eliminated the stimulation of proliferation and ALP activity of MC3T3-E1 which was induced by HJ. These results indicate that HJ directly stimulates cell proliferation and differentiation of osteoblasts. Also, when we assessed the effects of HJ on osteoblastic differentiation in MC3T3-E1, HJ enhanced ALP activity and mineralization in a dose- and time-dependent fashion. This stimulatory effect of the HJ was observed at relatively low doses (significant at 20-100 μg/ml and maximal at 100 μg/ml). Northern blot analysis showed that the HJ (60 μg/ml) increased in bone morphogenetic protein-2 as well as ALP mRNA concentrations in MC3T3-E1 cells. HJ (100 μg/ml) slightly increased in type I collagen mRNA abundance throughout the culture period, whereas it markedly inhibited the gene expression of collagenase-1 between days 15 and 20 of culture. These results indicate that HJ has anabolic effect on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of common metabolic bone diseases.-
dc.publisherElsevier-
dc.titleEffect of safflower seeds supplementation on stimulation of the proliferation, differentiation and mineralization of osteoblastic MC3T3-E1 cells-
dc.title.alternativeEffect of safflower seeds supplementation on stimulation of the proliferation, differentiation and mineralization of osteoblastic MC3T3-E1 cells-
dc.typeArticle-
dc.citation.titleJournal of Ethnopharmacology-
dc.citation.number1-
dc.citation.endPage49-
dc.citation.startPage42-
dc.citation.volume115-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.alternativeName김경운-
dc.contributor.alternativeName서숙종-
dc.contributor.alternativeName이태균-
dc.contributor.alternativeName하기태-
dc.contributor.alternativeName김준기-
dc.contributor.alternativeName김경훈-
dc.contributor.alternativeName김동일-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeName문태철-
dc.contributor.alternativeName김철호-
dc.identifier.bibliographicCitationJournal of Ethnopharmacology, vol. 115, no. 1, pp. 42-49-
dc.identifier.doi10.1016/j.jep.2007.09.003-
dc.subject.keywordAlkaline phosphatase-
dc.subject.keywordBone morphogenetic protein-2-
dc.subject.keywordCollagenase-1-
dc.subject.keywordDifferentiation-
dc.subject.keywordHonghwain-Jahage-
dc.subject.keywordMineralization-
dc.subject.keywordOsteoblast-
dc.subject.keywordProliferation-
dc.subject.keywordProstaglandin E2-
dc.subject.localAlkaline phosphatase-
dc.subject.localalkaline phosphatase-
dc.subject.localBone morphogenetic protein-2-
dc.subject.localCollagenase-1-
dc.subject.localdifferentiation-
dc.subject.localDifferentiation-
dc.subject.localHonghwain-Jahage-
dc.subject.localMineralization-
dc.subject.localOsteoblast-
dc.subject.localosteoblast-
dc.subject.localosteoblasts-
dc.subject.localOsteoblasts-
dc.subject.localproliferation-
dc.subject.localProliferation-
dc.subject.localprostaglandin E2-
dc.subject.localProstaglandin E2-
dc.subject.localProstaglandin e2-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.