Sphingosinicella soli sp. nov., isolated from an alkaline soil in Korea

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, KSL-125T was isolated from an alkaline soil from KwangcHun, Korea, and its taxonomic position was investigated in a polyphasic study. Strain KSL-125T grew optimally at 30 °C, at pH 7.5-8.0 and in the presence of 0.5% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA,gene sequences showed that strain KSL-125T joins the cluster comprising the two Sphingosinicella species at a bootstrap resampling value of 100 %. The values for similarity between the 16S RNA gene sequence of strain KSL-125T and those of the type strains of the two Sphingosinicella species were 98.9-99.0 %. Strain KSL-125T contained Q-10 as the predominant ubiquinone and C17:1ω6c, C16:1ω7c and/or iso-C15:0 2-OH and Cls1B:1ω7c as the major fatty acids. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyimonomethylethanolamine and an unidentified phospholipid. The DNA G + C content was 65.1 mol%. Strain KSL-125T was distinguishable from the two recognized Sphingosinicella species on the basis of differential phenotypic properties, DNA-DNA relatedness data and repetitive-sequence-based PCR genomic fingerprinting patterns. The phenotypic, phylogenetic and genetic data showed that strain KSL-125T represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella soli sp. nov. is proposed. The type strain is KSL-125T (=KCTC 12482T =DSM 17328T).