Daeguia caeni gen. nov., sp. nov., isolated from sludge of a textile dye works

Abstract

A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K107T, was isolated from sludge collected from a textile dye works in Korea and its taxonomic position was investigated by means of a polyphasic analysis. Strain K107T contained Q-10 as the predominant ubiquinone. The major fatty acids (>1 0% of total fatty acids) were C18, 1ω7c and C18:1 2-OH. The DNA G + C content was 57.0 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain K107T was closely related to the genera Mycoplana, Brucella and Ochrobactrum. Strain K107T exhibited 16S rRNA gene sequence similarity values of 96.3-97.1 % with respect to the type strains of two Mycoplana species and 94.8-96.8 % with respect to members of the genera Brucella and Ochrobactrum. A phylogenetic analysis based on recA gene sequences showed that strain K107T forms a distinct phylogenetic lineage within the Alphaproteobacteria. The recA gene sequence of strain K107T showed similarity values of 84.5 % with respect to type strains of Brucella species and values of 77.6-83.1 % with respect to members of the genera Pseudochrobactrum, Ochrobactrum and Mycoplana. Strain K107T could be differentiated from phylogenetically related genera by differences in phenotypic properties and fatty acid profiles. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain K107T represents a novel genus and species, for which the name Daeguia caeni gen. nov., sp. nov. is proposed. The type strain of Daeguia caeni is strain K107T (=KCTC 12981T=CCUG 54520T).