Expression of CP4 5-Enol-Pyruvylshikimate-3-Phosphate synthase transgene in inbred line of Korean domestic maize(Zea may L.) = 국내 옥수수 순계주에서 CP4 5-Enol-Pyruvylshikimate-3-Phosphate synthase 유전자의 발현

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dc.contributor.authorM A Cho-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorJ S Kim-
dc.contributor.authorB K Lee-
dc.contributor.authorC Y Moon-
dc.contributor.authorP S Choi-
dc.date.accessioned2017-04-19T09:08:53Z-
dc.date.available2017-04-19T09:08:53Z-
dc.date.issued2007-
dc.identifier.issn1229-2818-
dc.identifier.uri10.5010/JPB.2007.34.4.375ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8251-
dc.description.abstractThis study was conducted to develop herbicide-resistance domestic maize plants by introducing the CP4 5-enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) gene using Agrobacterium tumefaciensmediated immature embryo transformation. Immature embryos of five genotypes (HW1, KL103, HW3, HW4, HW7) were co-cultivated with strains Agrobacterium tumefaciens (strain C58C1) containing the binary vector (pCAMBIA2300) carrying Ubiquitin promoter-CP4 EPSPS gene and Cauliflower mosaic virus 35S (CaMV35S) promoter-nptII gene conferring resistance to paromomycin as a selective agent. The presence and expression of CP4 EPSPS transgene were confirmed by PCR, RT-PCR and Northern blot analysis, respectively. Also, the resistance to glyphosate in the transgenic maize (T1) was analyzed by shikimate accumulation assay. The frequency (%) of paromomycin-resistance callus was 0.37, 0.03, 2.20, 2.37, and 0.81% in pure lines HW1, KL103, HW3, HW4 and HW7, respectively. EPSP transgene sequences were amplified in putative transgenic plants that regenerated from paromomycin-resistance calli of two inbred lines (HW3, HW4). of them, RT-PCR and Northern blot analyses revealed that the transgene was only expressed in two transgenic events (M266, M104) of HW4 inbred line, and a mild glyphosate resistance of transgenic event (M266) was confirmed by the lower shikimate accumulation in leaf segments. These results demonstrate that transgenic maize with herbicide-resistance traits in Korean genotype can be genetically obtained.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression of CP4 5-Enol-Pyruvylshikimate-3-Phosphate synthase transgene in inbred line of Korean domestic maize(Zea may L.) = 국내 옥수수 순계주에서 CP4 5-Enol-Pyruvylshikimate-3-Phosphate synthase 유전자의 발현-
dc.title.alternativeExpression of CP4 5-Enol-Pyruvylshikimate-3-Phosphate synthase transgene in inbred line of Korean domestic maize(Zea may L.)-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number4-
dc.citation.endPage380-
dc.citation.startPage375-
dc.citation.volume34-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.alternativeName조미애-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName김진석-
dc.contributor.alternativeName이병규-
dc.contributor.alternativeName문추연-
dc.contributor.alternativeName최필선-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 34, no. 4, pp. 375-380-
dc.identifier.doi10.5010/JPB.2007.34.4.375-
dc.description.journalClassN-
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