Constitutive coexpression of Bacillus endoxylanase and Trichoderma endoglucanase genes in Saccharomyces cerevisiae

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dc.contributor.authorJ H Lee-
dc.contributor.authorM Y Lim-
dc.contributor.authorM J Kim-
dc.contributor.authorSun Yeon Heo-
dc.contributor.authorJ H Seo-
dc.contributor.authorY H Kim-
dc.contributor.authorS W Nam-
dc.date.accessioned2017-04-19T09:08:53Z-
dc.date.available2017-04-19T09:08:53Z-
dc.date.issued2007-
dc.identifier.issn10177825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8253-
dc.description.abstractThe endoxylanase (GenBank Access No. U51675) of Bacillus spp. and endoglucanase (GenBank Access No. AY466436) of Trichoderma spp. were separately inserted downstream of the yeast constitutive ADH1 promoter, resulting in three different plasmids (pAGX1, pAGX2, and pAGX3) according to the transcription direction of two genes. When the yeast transformants, S. cerevisiae SEY2102 harboring each expression plasmid, were grown on YPD medium, the total activities of the enzymes were approximately 3.01 unit/ml, 3.24 unit/ml, and 7.56 unit/ml for endoxylanase and 0.60 unit/ml, 0.54 unit/ml, and 0.39 unit/ml for endoglucanase, in the following order: the pAGX1, pAGX2, and pAGX3. More than 70% of the endoxylanase and endoglucanase activities was found in the extracellular media.-
dc.publisherSouth Korea-
dc.titleConstitutive coexpression of Bacillus endoxylanase and Trichoderma endoglucanase genes in Saccharomyces cerevisiae-
dc.title.alternativeConstitutive coexpression of Bacillus endoxylanase and Trichoderma endoglucanase genes in Saccharomyces cerevisiae-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number12-
dc.citation.endPage2080-
dc.citation.startPage2076-
dc.citation.volume17-
dc.contributor.alternativeName이재형-
dc.contributor.alternativeName임명예-
dc.contributor.alternativeName김미진-
dc.contributor.alternativeName허선연-
dc.contributor.alternativeName서진호-
dc.contributor.alternativeName김연희-
dc.contributor.alternativeName남수완-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 17, no. 12, pp. 2076-2080-
dc.subject.keywordcoexpression-
dc.subject.keywordendoglucanase-
dc.subject.keywordendoxylanase-
dc.subject.keywordS. cerevisiae-
dc.subject.keywordxylan-
dc.subject.localcoexpression-
dc.subject.localCoexpression-
dc.subject.localendoglucanase-
dc.subject.localEndoglucanase-
dc.subject.localendoxylanase-
dc.subject.localEndoxylanase-
dc.subject.localS. cerevisiae-
dc.subject.localxylan-
dc.description.journalClassY-
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