Screening of the genes expressed in Pichia pastoris grown in phosphate-limited chemostat culture = 인산제한상태에서 발현되는 Pichia pastoris 유래 유전자 탐색

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJi Yeon Hong-
dc.contributor.authorJungoh Ahn-
dc.contributor.authorM S Park-
dc.contributor.authorS Y Choi-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorJoon Ki Jung-
dc.contributor.authorHong-Weon Lee-
dc.date.accessioned2017-04-19T09:08:54Z-
dc.date.available2017-04-19T09:08:54Z-
dc.date.issued2007-
dc.identifier.issn0257-2389-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8254-
dc.description.abstractThe physiological responses of microorganisms to specific nutrient limitation can be regulated at the transcriptional levels. In this study, in order to develop the Pichia pastoris-derived promoter inducible by nutrient-limited condition, we constructed cDNA libraries using RT-PCR of total RNA from P. pastoris in steady-states of phosphate-limited chemostat with different dilution rates. Various genes were detected from cDNA library. Among these genes, the gene encoding putative sodium/phosphate (Na+/Pi) symporter (NPS), high affinity transporter of phosphate, was detected. It was observed that expression of NPS increased in a manner specific to phosphate-limited condition through Northern blot. Therefore, it is thought that the promoter from NPS gene may have the potential as auto-inducible promoter by phosphate-limited culture condition without inducer.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleScreening of the genes expressed in Pichia pastoris grown in phosphate-limited chemostat culture = 인산제한상태에서 발현되는 Pichia pastoris 유래 유전자 탐색-
dc.title.alternativeScreening of the genes expressed in Pichia pastoris grown in phosphate-limited chemostat culture-
dc.typeArticle-
dc.citation.titleKorean Journal of (Applied) Microbiology & Biotechnology-
dc.citation.number4-
dc.citation.endPage277-
dc.citation.startPage272-
dc.citation.volume35-
dc.contributor.affiliatedAuthorJi Yeon Hong-
dc.contributor.affiliatedAuthorJungoh Ahn-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.affiliatedAuthorJoon Ki Jung-
dc.contributor.affiliatedAuthorHong-Weon Lee-
dc.contributor.alternativeName홍지연-
dc.contributor.alternativeName안정오-
dc.contributor.alternativeName박명수-
dc.contributor.alternativeName최순용-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName정준기-
dc.contributor.alternativeName이홍원-
dc.identifier.bibliographicCitationKorean Journal of (Applied) Microbiology & Biotechnology, vol. 35, no. 4, pp. 272-277-
dc.subject.keywordphosphate limitation-
dc.subject.keywordpichia pastoris-
dc.subject.keywordsodium-phosphate (Na+/Pi) symporter (NPS)-
dc.subject.localphosphate limitation-
dc.subject.localpichia pastoris-
dc.subject.localPichia pastoris-
dc.subject.localsodium-phosphate (Na+/Pi) symporter (NPS)-
dc.description.journalClassN-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.