Expression of human NDRG2 by myeloid dendritic cells inhibits down-regulation of activated leukocyte cell adhesion molecule (ALCAM) and contributes to maintenance of T cell stimulatory activity

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dc.contributor.authorSeung-Chul Choi-
dc.contributor.authorK D Kim-
dc.contributor.authorJong-Tae Kim-
dc.contributor.authorJae Wha Kim-
dc.contributor.authorHee Gu Lee-
dc.contributor.authorJ M Kim-
dc.contributor.authorY S Jang-
dc.contributor.authorD Y Yoon-
dc.contributor.authorK I Kim-
dc.contributor.authorY Yang-
dc.contributor.authorD H Cho-
dc.contributor.authorJ S Lim-
dc.date.accessioned2017-04-19T09:08:55Z-
dc.date.available2017-04-19T09:08:55Z-
dc.date.issued2008-
dc.identifier.issn0741-5400-
dc.identifier.uri10.1189/jlb.0507300ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8264-
dc.description.abstractWe reported previously that N-myc downstream-regulated gene 2 (NDRG2), a member of a new family of differentiation-related genes, is expressed specifically in dendritic cells (DC) differentiated from monocytes, CD34 + progenitor cells, and the myelomonocytic leukemic cell line. In this study, we demonstrate that NDRG2 protein expression is detected, not only in in vitro-differentiated DC but also in primary DC from lymph nodes, thymus, and skin when anti-NDRG2 antibodies are used. As predicted from previous studies investigating the mRNA expression pattern of several types of cell lines, progenitor cells, and DC, NDRG2 protein was expressed strongly in DC. Its expression was detected at significant levels after differentiation from progenitor cells. RNA interference of NDRG2 demonstrated that activated leukocyte cell adhesion molecule (ALCAM) expression is down-regulated specifically in DC differentiated from NDRG2 small interfering RNA (siRNA)-transfected monocytes. This was consistent with our observation that U937 cells transfected with NDRG2 became resistant to the GM-CSF/IL-4-induced ALCAM reduction. Furthermore, DC, which had differentiated from NDRG2 siRNA-transfected monocytes, showed a reduced ability to induce T cell proliferation. Taken together, our results indicate that NDRG2 is able to preserve ALCAM expression during DC differentiation from monocytes under cytokine culture conditions and that its expression helps DC maintain costimulatory signals necessary for T cell stimulation.-
dc.publisherWiley-
dc.titleExpression of human NDRG2 by myeloid dendritic cells inhibits down-regulation of activated leukocyte cell adhesion molecule (ALCAM) and contributes to maintenance of T cell stimulatory activity-
dc.title.alternativeExpression of human NDRG2 by myeloid dendritic cells inhibits down-regulation of activated leukocyte cell adhesion molecule (ALCAM) and contributes to maintenance of T cell stimulatory activity-
dc.typeArticle-
dc.citation.titleJournal of Leukocyte Biology-
dc.citation.number1-
dc.citation.endPage98-
dc.citation.startPage89-
dc.citation.volume83-
dc.contributor.affiliatedAuthorSeung-Chul Choi-
dc.contributor.affiliatedAuthorJong-Tae Kim-
dc.contributor.affiliatedAuthorJae Wha Kim-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName최승철-
dc.contributor.alternativeName김광동-
dc.contributor.alternativeName김종태-
dc.contributor.alternativeName김재화-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeName김진만-
dc.contributor.alternativeName장용석-
dc.contributor.alternativeName윤도영-
dc.contributor.alternativeName김근일-
dc.contributor.alternativeName양영-
dc.contributor.alternativeName조대호-
dc.contributor.alternativeName임종석-
dc.identifier.bibliographicCitationJournal of Leukocyte Biology, vol. 83, no. 1, pp. 89-98-
dc.identifier.doi10.1189/jlb.0507300-
dc.subject.keywordDendritic cells (DC)-
dc.subject.keywordsiRNA-
dc.subject.localDendritic cell-
dc.subject.localDendritic cells (DC)-
dc.subject.localdendritic cells-
dc.subject.localDendritic cells (DCs)-
dc.subject.localDendritic cells-
dc.subject.localdendritic cells (DC)-
dc.subject.localdendritic cell-
dc.subject.localsiRNA-
dc.subject.localSiRNA-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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