Hydrogenophaga bisanensis sp. nov., isolated from wastewater of a textile dye works

Abstract

A Gram-negative, non-spore-forming, rod-shaped, Hydrogenophaga-like bacterial strain, K102T, was isolated from wastewater collected from a textile dye works in Korea and subjected to a polyphasic taxonomic study. Strain K102T grew optimally at pH 7.0-8.0 and 30-37 °C in the presence of 0.5% (w/v) NaCl. It contained O-8 as the predominant ubiquinone and C16:0 C16:1 ω7c and/or iso-C15:0 2-OH and C18:1 ω7c as the major fatty acids. The DNA G + C content was 64.8 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA-gene sequences showed that strain K102T fell within the radiation of the cluster comprising species of the genus Hydrogenophaga. Strain K102T exhibited 16S rRNA gene sequence similarity values of 96.9-98.9% to the type strains of recognized Hydrogenoohaga species. Levels of DNA-DNA relatedness between strain K102T and the type strains of its four phylogenetically most closely related species, together with differential phenotypic properties, revealed that strain K102T could be distinguished from all recognized species of the genus Hydrogenophaga. On the basis of phenotypic, phylogenetic and genetic data, strain K102T is considered to represent a novel species of the genus Hydrogenophaga, for which the name Hydrogenophaga bisanensis sp. nov. is proposed. The type strain is K102T (=KCTC 12980T =CCUG 54518T).