Controlled antibody immobilization onto immunoanalytical platforms by synthetic peptide

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dc.contributor.authorYongwon Jung-
dc.contributor.authorH J Kang-
dc.contributor.authorJeong Min Lee-
dc.contributor.authorS O Jung-
dc.contributor.authorW S Yun-
dc.contributor.authorSang Jeon Chung-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:08:57Z-
dc.date.available2017-04-19T09:08:57Z-
dc.date.issued2008-
dc.identifier.issn0003-2697-
dc.identifier.uri10.1016/j.ab.2007.10.022ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8277-
dc.description.abstractAntibody immobilization on a solid surface is inevitable in the preparation of immunochips/sensors. Antibody-binding proteins such as proteins A and G have been extensively employed to capture antibodies on sensor surfaces with right orientations, maintaining their full functionality. Because of their synthetic versatility and stability, in general, small molecules have more advantages than proteins. Nevertheless, no small molecule has been used for oriented and specific antibody immobilization. Here is described a novel strategy to immobilize an antibody on various sensor surfaces by using a small antibody-binding peptide. The peptide binds specifically to the Fc domain of immunoglobulin G (IgG) and, therefore, affords a properly oriented antibody surface. Surface plasmon resonance analysis indicated that a peptide linked to a gold chip surface through a hydrophilic linker efficiently captured human and rabbit IgGs. Moreover, antibodies captured by the peptide exhibited higher antigen binding capacity compared with randomly immobilized antibodies. Peptide-mediated antibody immobilization was successfully applied on the surfaces of biosensor substrates such as magnetic particles and glass slides. The antibody-binding peptide conjugate introduced in this work is the first small molecule linker that offers a highly stable and specific surface platform for antibody immobilization in immunoassays.-
dc.publisherElsevier-
dc.titleControlled antibody immobilization onto immunoanalytical platforms by synthetic peptide-
dc.title.alternativeControlled antibody immobilization onto immunoanalytical platforms by synthetic peptide-
dc.typeArticle-
dc.citation.titleAnalytical Biochemistry-
dc.citation.number1-
dc.citation.endPage105-
dc.citation.startPage99-
dc.citation.volume374-
dc.contributor.affiliatedAuthorYongwon Jung-
dc.contributor.affiliatedAuthorH J Kang-
dc.contributor.affiliatedAuthorJeong Min Lee-
dc.contributor.affiliatedAuthorSang Jeon Chung-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName정용원-
dc.contributor.alternativeName강효진-
dc.contributor.alternativeName이정민-
dc.contributor.alternativeName정선옥-
dc.contributor.alternativeName윤완수-
dc.contributor.alternativeName정상전-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationAnalytical Biochemistry, vol. 374, no. 1, pp. 99-105-
dc.identifier.doi10.1016/j.ab.2007.10.022-
dc.subject.keywordAntibody immobilization-
dc.subject.keywordBiochip-
dc.subject.keywordBiosensor-
dc.subject.keywordFc-binding peptide-
dc.subject.keywordImmunoassay-
dc.subject.localAntibody Immobilization-
dc.subject.localAntibody immobilization-
dc.subject.localantibody immobilization-
dc.subject.localBiochip-
dc.subject.localbiochip-
dc.subject.localbiosensor-
dc.subject.localBio-sensor-
dc.subject.localBiosensor-
dc.subject.localbiosensors-
dc.subject.localBiosensors-
dc.subject.localFc-binding peptide-
dc.subject.localImmunoassay-
dc.subject.localImmunoassays-
dc.subject.localimmunoassay-
dc.description.journalClassY-
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