Structural basis for the cold adaptation of psychrophilic M37 lipase from Photobacterium lipolyticum = Photobacterium lipolyticum에서 유래한 호냉성 M37리파아제의 저온적응에 대한 구조적인 이해

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Title
Structural basis for the cold adaptation of psychrophilic M37 lipase from Photobacterium lipolyticum = Photobacterium lipolyticum에서 유래한 호냉성 M37리파아제의 저온적응에 대한 구조적인 이해
Author(s)
Suk Kyeong Jung; Dae Gwin Jeong; M S Lee; J K Lee; H K Kim; Seong Eon Ryu; Byoung Chul Park; J H Kim; Seung Jun Kim
Bibliographic Citation
Proteins-Structure Function and Bioinformatics, vol. 71, no. 1, pp. 476-484
Publication Year
2008
Abstract
The M37 lipase from Photobacterium lipolyticum shows an extremely low activation energy and strong activity at low temperatures, with optimum activity seen at 298 K and more than 75% of the optimum activity retained down to 278 K. Though the M37 lipase is most closely related to the filamentous fungal lipase, Rhizomucor miehei lipase (RML) at the primary structure level their activity characteristics are completely different. In an effort to identify structural components of cold adaptation in lipases, we determined the crystal structure of the M37 lipase at 2.2 ? resolution and compared it to that of nonadapted RML. Structural analysis revealed that M37 lipase adopted a folding pattern similar to that observed for other lipase structures. However, comparison with RML revealed that the region beneath the lid of the M37 lipase included a significant and unique cavity that would be occupied by a lid helix upon substrate binding. In addition, the oxyanion hole was much wider in M37 lipase than RML. We propose that these distinct structural characteristics of M37 lipase may facilitate the lateral movement of the helical lid and subsequent substrate hydrolysis, which might explain its low activation energy and high activity at low temperatures.
Keyword
cavitycold adaptationM37 lipaseoxyanion holerhizomucor miehei lipase (RML)
ISSN
0887-3585
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/prot.21884
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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