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- Title
- Proteomic analysis of the proteins regulated by HrpB from the plant pathogenic bacterium Burkholderia glumae
- Author(s)
- Y Kang; J Kim; S Kim; H Kim; J Y Lim; M Kim; Jangryul Kwak; Jae Sun Moon; I Hwang
- Bibliographic Citation
- Proteomics, vol. 8, no. 1, pp. 106-121
- Publication Year
- 2008
- Abstract
- plant cells via the hypersensitive response and pathogenicity (Hrp) type III protein secretion system (T3SS) during infection. The genes encoding the Hrp T3SS are expressed only under plant apoplast-mimicking conditions in an AraC-type transcriptional activator HrpB-dependent manner. To identify the proteins controlled by HrpB in Burkholderia glumae in vitro, we constitutively expressed hrpB and analyzed the proteins showing altered expression using 2-DE and ESI-MS/MS. Among 46 proteins exhibiting consistently altered expression, which were encoded by 34 different genes, 34 were secretory proteins and 12 were cytoplasmic. Twenty-eight of the secreted proteins showed increased accumulation, whereas the other six showed decreased accumulation. None of the HrpB-dependent proteins had significant homology to known T3SS-dependent proteins, except for HrpK from Pseudomonas syringae pv. syringae and two T3SS-associated cytoplasmic proteins from Ralstonia solanacearum. Twenty-one of the 34 genes had putative HrpB-binding sequences in their upstream regulatory regions. Secretion of all 34 extracellular proteins was independent of the Hrp T3SS, and 16 were secreted via a type II protein secretion system (T2SS). Mutants lacking the T2SS or Hrp T3SS produced toxoflavin but were less virulent to rice panicles, indicating the importance of these proteins in pathogenicity.
- Keyword
- burkholderia glumaeHrpBsecretiontwo-dimensional electrophoresis
- ISSN
- 1615-9853
- Publisher
- Wiley
- DOI
- http://dx.doi.org/10.1002/pmic.200700244
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
- Files in This Item:
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