Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis

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Title
Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis
Author(s)
Y S Shin; K H Bang; D S In; O T Kim; D Y Hyun; I O Ahn; B C Ku; Suk Weon Kim; N S Seong; S W Cha; D Lee; H K Choi
Bibliographic Citation
Archives of Pharmacal Research, vol. 30, no. 12, pp. 1625-1628
Publication Year
2007
Abstract
Fingerprinting analysis of fresh ginseng according to root age was performed using 1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the 1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large 1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of 1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.
Keyword
1H-NMR spectroscopyFingerprinting analysisGinseng rootPrincipal component analysis
ISSN
0253-6269
Publisher
Pharmaceutical Soc Korea
DOI
http://dx.doi.org/10.1007/BF02977333
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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