Genome-wide drug-induced haploinsufficiency screening of fission yeast for identification of hydrazinocurcumin targets

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dc.contributor.authorSeung-Tae Baek-
dc.contributor.authorDong Uk Kim-
dc.contributor.authorS Han-
dc.contributor.authorI S Woo-
dc.contributor.authorMiyoung Nam-
dc.contributor.authorL Kim-
dc.contributor.authorKyung Sun Heo-
dc.contributor.authorHye Mi Lee-
dc.contributor.authorH R Hwang-
dc.contributor.authorShin Jung Choi-
dc.contributor.authorMi Sun Won-
dc.contributor.authorM Lee-
dc.contributor.authorSong Kyu Park-
dc.contributor.authorS Lee-
dc.contributor.authorH J Kwon-
dc.contributor.authorP J Maeng-
dc.contributor.authorH M Park-
dc.contributor.authorYoung Woo Park-
dc.contributor.authorD Kim-
dc.contributor.authorKwang Lae Hoe-
dc.date.accessioned2017-04-19T09:09:37Z-
dc.date.available2017-04-19T09:09:37Z-
dc.date.issued2008-
dc.identifier.issn10177825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8318-
dc.description.abstractHydrazinocurcumin (HC), a synthetic derivative of curcumin, has been reported to inhibit angiogenesis via unknown mechanisms. Understanding the molecular mechanisms of the drug's action is important for the development of improved compounds with better pharmacological properties. A genome-wide drug-induced haploinsufficiency screening of fission yeast gene deletion mutants has been applied to identify drug targets of HC. As a first step, the 50% inhibition concentration (IG50) of HC was determined to be 2.2 μM. The initial screening of 4,158 mutants in 384-well plates using robotics was performed at concentrations of 2,3, and 4 μM. A second screening was performed to detect sensitivity to HC on the plates. The first screening revealed 178 candidates, and the second screening resulted in 13 candidates, following the elimination of 165 false positives. Final filtering of the condition-dependent haploinsufficient genes gave eight target genes. Analysis of the specific targets of HC has shown that they are related to septum formation and the general transcription processes, which may be related to histone acetyltransferase. The target mutants showed 65% growth inhibition in response to HC compared with wild-type controls, as shown by liquid culture assay.-
dc.publisherSouth Korea-
dc.titleGenome-wide drug-induced haploinsufficiency screening of fission yeast for identification of hydrazinocurcumin targets-
dc.title.alternativeGenome-wide drug-induced haploinsufficiency screening of fission yeast for identification of hydrazinocurcumin targets-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number2-
dc.citation.endPage269-
dc.citation.startPage263-
dc.citation.volume18-
dc.contributor.affiliatedAuthorDong Uk Kim-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.alternativeName백승태-
dc.contributor.alternativeName김동욱-
dc.contributor.alternativeName한상조-
dc.contributor.alternativeName우임선-
dc.contributor.alternativeName남미영-
dc.contributor.alternativeName김리라-
dc.contributor.alternativeName허경선-
dc.contributor.alternativeName이혜미-
dc.contributor.alternativeName황혜림-
dc.contributor.alternativeName최신정-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName이민호-
dc.contributor.alternativeName박성규-
dc.contributor.alternativeName이성호-
dc.contributor.alternativeName권호정-
dc.contributor.alternativeName맹필제-
dc.contributor.alternativeName박희문-
dc.contributor.alternativeName박영우-
dc.contributor.alternativeName김동섭-
dc.contributor.alternativeName허광래-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 18, no. 2, pp. 263-269-
dc.subject.keywordhaploinsufficiency-
dc.subject.keywordhydrazinocurcumin-
dc.subject.keywordseptin-
dc.subject.keywordtaf4 yeast-
dc.subject.localhaploinsufficiency-
dc.subject.localhydrazinocurcumin-
dc.subject.localseptin-
dc.subject.localtaf4 yeast-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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