Epigenetic down-regulation and suppressive role of DCBLD2 in gastric cancer cell proliferation and invasion

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Title
Epigenetic down-regulation and suppressive role of DCBLD2 in gastric cancer cell proliferation and invasion
Author(s)
Mirang Kim; K T Lee; Hay-Ran Jang; Jeong Hwan Kim; S M Noh; K S Song; J S Cho; H Y Jeong; Seon-Young Kim; Hyang Sook Yoo; Yong Sung Kim
Bibliographic Citation
Molecular Cancer Research, vol. 6, no. 2, pp. 222-230
Publication Year
2008
Abstract
The promoter region of Discoidin, CUB and LCCL domain containing 2 (DCBLD2) was found to be aberrantly methylated in gastric cancer cell lines and in primary gastric cancers, as determined by restriction landmark genomic scanning. DCBLD2 expression was inversely correlated with DCBLD2 methylation in gastric cancer cell lines. Treatment with 5-aza-2′-deoxycytidine and trichostatin A partially reversed DCBLD2 methylation and restored gene expression in DCBLD2-silenced cell lines. In an independent series of 82 paired gastric cancers and adjacent normal tissues, DCBLD2 expression was down-regulated in 79% of gastric cancers as compared with normal tissues as measured by real-time reverse transcription-PCR. Pyrosequencing analysis of the DCBLD2 promoter region revealed abnormal hypermethylation in gastric cancers, and this hypermethylation was significantly correlated with down-regulation of DCBLD2 expression. Furthermore, ectopic expression of DCBLD2 in gastric cancer cell lines inhibited colony formation in both anchorage-dependent and anchorage-independent cultures and also inhibited invasion through the collagen matrix. These data suggest that down-regulation of DCBLD2, often associated with promoter hypermethylation, is a frequent event that may be related to the development of gastric cancer.
ISSN
1541-7786
Publisher
Amer Assoc Cancer Research
DOI
http://dx.doi.org/10.1158/1541-7786.MCR-07-0142
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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