Ionizing radiation can induce GSK-3β phosphorylation and NF-κB transcriptional transactivation in ATM-deficient fibroblasts

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Title
Ionizing radiation can induce GSK-3β phosphorylation and NF-κB transcriptional transactivation in ATM-deficient fibroblasts
Author(s)
Osong Kwon; K A Kim; L He; S O Kim; Min-Soo Kim; Eun-Young Cha; Byung Dae Yoon; D E Sok; M Jung; Jong Seog AhnBo Yeon Kim
Bibliographic Citation
Cellular Signalling, vol. 20, no. 4, pp. 602-612
Publication Year
2008
Abstract
DNA damage by ionizing radiation (IR) can induce activations of both NF-κB and p53 through the upstream kinase ataxia telangiectasia mutated (ATM). NF-κB activation could also be signaled through two distinct or overlapped pathways; IκB kinases (IKKs)-IκBα and Akt-glycogen synthase kinase-3 (GSK-3). In the present study, however, we show that activation of Akt1 and the subsequent phosphorylation and inactivation of GSK-3β by IR could also occur in ATM-deficient AT5BIVA cells as well as in normal MRC5CV1 fibroblasts. Similarly, lithium chloride (LiCl) was found to increase the phosphorylation of GSK-3β independently of ATM. Transfection with either wild-type or kinase dead mutant GSK-3β to the cells further indicated that phosphorylations of Akt1 and GSK-3β were closely associated with the transcriptional transactivation of NF-κB in response to ionizing radiation. On the other hand, LiCl, having no effect on caspase-3 activation, significantly increased p53 phosphorylation and apoptotic death of the normal MRC5CV1 cells while IR, activating both caspase-3 and p53, profoundly affected AT5BIVA cell death. Hence, our data suggest that although ATM-mediated IKK-IκBα pathway might be a typical pathway for IR-induced NF-κB activation, GSK-3β phosphorylation could also partially contribute to the transcriptional transactivation of NF-κB in an ATM-independent manner and that GSK-3β phosphorylation could induce ATM-mediated cell apoptosis through the activation of p53.
Keyword
AktAtaxia telangiectasiaGSK-3Ionizing radiation
ISSN
0898-6568
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.cellsig.2007.10.022
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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