Molecular characterization of a cDNA encoding DRE-binding transcription factor from dehydration-treated fibrous roots of sweetpotato

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Title
Molecular characterization of a cDNA encoding DRE-binding transcription factor from dehydration-treated fibrous roots of sweetpotato
Author(s)
Yun Hee Kim; Kyoung Sil Yang; S H Ryu; K Y Kim; W K Song; Suk Yoon Kwon; Haeng Soon Lee; J W Bang; Sang Soo Kwak
Bibliographic Citation
Plant Physiology and Biochemistry, vol. 46, no. 2, pp. 196-204
Publication Year
2008
Abstract
A new dehydration responsive element-binding (DREB) protein gene encoding for an AP2/EREBP-type transcription factor was isolated by screening of the cDNA library for dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). Its cDNA (referred to as swDREB1) fragment of 1206 bp was sequenced from, which a 257 amino acid residue protein was deduced with a predicted molecular weight of 28.17 kDa. A search of the protein BLAST database revealed that this protein can be classified as a typical member of a DREB subfamily. RT-PCR and northern analyses revealed diverse expression patterns of the swDREB1 gene in various tissues of intact sweetpotato plant, and in leaves and fibrous roots exposed to different stresses. The swDREB1 gene was highly expressed in stems and tuberous roots. In fibrous roots, its mRNA accumulation profiles clearly showed strong expression under various abiotic stress conditions such as dehydration, chilling, salt, methyl viologen (MV), and cadmium (Cd) treatment, whereas it did not respond to abscisic acid (ABA) or copper (Cu) treatment. The above results indicate that swDREB1 may be involved in the process of the plant response to diverse abiotic stresses through an ABA-independent pathway.
Keyword
Abscisic acidDehydrationDREBFibrous rootSweetpotato
ISSN
0981-9428
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.plaphy.2007.09.012
Type
Article
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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