Halobacillus seohaensis sp. nov., isolated from a marine solar saltern in Korea

Abstract

A Gram-positive or Gram-variable, motile, modarately halophilic, rod-shaped bacterial strain, ISL-50T, was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic study. Strain ISL-50T grew optimally at pH 7.0-8.0 and 37 °C in the presence of 8% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-50T belonged to the genus Halobacillus. Strain ISL-50T exhibited 16S rRNA gene sequence similarity values of 95.3-98.2.% with respect to the type strains of recognized Halobacillus species. The cell-wall peptidoglycan'was based on meso-diaminopimelic acid, the predominant menaquinone was MK-7 and the major fatty acids were anteiSp-C15:0 and anteiso-C17:0. The DNA G + C content was 39.3 mol%. DNA-DNA relatedness data and differential phenotypic properties revealed that strain ISL-50T was distinguishable from the recognized Halobacillus species. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain ISL-50T represents a novel species of the genus Halobacillus, for which the name Halobacillus seohaensis sp. nov. is proposed. The type strain is ISL-50T (=KCTC 13145T=CCUG 54358T).