Chryseobacterium aquifrigidense sp. nov., isolated from a water-cooling system

Abstract

A non-motile, yellow-pigmented bacterium, designated strain CW9T, was isolated from a water-cooling system in Gwangyang, Republic of Korea. The cells were Gram-negative, catalase- and oxidase-positive, short rods. The major fatty acids were iso-C15:0 (45.5%), iso-C17:1ω9c (14.4 %), iso-C17:0 3-OH (13.0 %) and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1ω7c; 8.8 %). The DNA G+C content was 35.6 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW9T formed a lineage within the genus Chryseobacterium and was closely related to Chryseobacterium gleum ATCC 35910T (98.4% sequence similarity) and Chryseobacterium indologenes ATCC 29897T (97.8% sequence similarity). Phenotypic characteristics and DNA-DNA relatedness data served to distinguish strain CW9T from these two species. On the basis of the evidence presented in this study, strain CW9T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aquifrigidense sp. nov. is proposed. The type strain is CW9T (=KCTC 12894T=JCM 14756T).