Terrein inhibits keratinocyte proliferation via ERK inactivation and G2/M cell cycle arrest

Cited 25 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorD S Kim-
dc.contributor.authorH K Lee-
dc.contributor.authorS H Park-
dc.contributor.authorSangku Lee-
dc.contributor.authorIn Ja Ryoo-
dc.contributor.authorWon Gon Kim-
dc.contributor.authorIck Dong Yoo-
dc.contributor.authorJ I Na-
dc.contributor.authorS B Kwon-
dc.contributor.authorK C Park-
dc.date.accessioned2017-04-19T09:09:51Z-
dc.date.available2017-04-19T09:09:51Z-
dc.date.issued2008-
dc.identifier.issn0906-6705-
dc.identifier.uri10.1111/j.1600-0625.2007.00646.xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8354-
dc.description.abstractTerrein, a fungal metabolite, has been recently shown to have a strong antiproliferative effect on skin equivalents. In the present study, we further investigated the effects of terrein on the possible signalling pathways involved in the growth inhibition of human epidermal keratinocytes by examining the regulations of extracellular signal-regulated protein kinase (ERK) and of the Akt pathway by terrein. It was observed that ERK was inactivated by terrein and that keratinocyte proliferation was inhibited, whereas Akt was unaffected. The inhibition of the ERK pathway by U0126 (a specific ERK inhibitor) also had a dose-dependent antiproliferative effect on human keratinocytes. These results indicate that ERK inhibition is involved in keratinocyte growth inhibition by terrein. Moreover, flow cytometric analysis showed that terrein inhibits DNA synthesis, as evidenced by a reduction in the S phase and an increase in the G2/M phase of the cell cycle. Thus, we next examined changes in the expressions of G2/M cell cycle-related proteins. Terrein was found to downregulate cyclin B1 and Cdc2 without Cdc2 phosphorylation, but upregulated p27KIP1 (p27), a known inhibitor of cyclin-dependent kinase. These results suggest that terrein reduces human keratinocyte proliferation by inhibiting ERK and by decreasing the expressions of cyclin B1 and Cdc2 complex.-
dc.publisherWiley-
dc.titleTerrein inhibits keratinocyte proliferation via ERK inactivation and G2/M cell cycle arrest-
dc.title.alternativeTerrein inhibits keratinocyte proliferation via ERK inactivation and G2/M cell cycle arrest-
dc.typeArticle-
dc.citation.titleExperimental Dermatology-
dc.citation.number4-
dc.citation.endPage317-
dc.citation.startPage312-
dc.citation.volume17-
dc.contributor.affiliatedAuthorSangku Lee-
dc.contributor.affiliatedAuthorIn Ja Ryoo-
dc.contributor.affiliatedAuthorWon Gon Kim-
dc.contributor.affiliatedAuthorIck Dong Yoo-
dc.contributor.alternativeName김동석-
dc.contributor.alternativeName이현경-
dc.contributor.alternativeName박세형-
dc.contributor.alternativeName이상구-
dc.contributor.alternativeName류인자-
dc.contributor.alternativeName김원곤-
dc.contributor.alternativeName유익동-
dc.contributor.alternativeName나정임-
dc.contributor.alternativeName권선방-
dc.contributor.alternativeName박경찬-
dc.identifier.bibliographicCitationExperimental Dermatology, vol. 17, no. 4, pp. 312-317-
dc.identifier.doi10.1111/j.1600-0625.2007.00646.x-
dc.subject.keywordcell cycle-
dc.subject.keywordERK-
dc.subject.keywordkeratinocytes-
dc.subject.keywordproliferation-
dc.subject.keywordterrein-
dc.subject.localcell cycle-
dc.subject.localCell cycle-
dc.subject.localERK-
dc.subject.localErk-
dc.subject.localkeratinocyte-
dc.subject.localKeratinocytes-
dc.subject.localKeratinocyte-
dc.subject.localkeratinocytes-
dc.subject.localproliferation-
dc.subject.localProliferation-
dc.subject.localterrein-
dc.subject.localTerrein-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.