Thermostable β-glycosidase-CBD fusion protein for biochemical analysis of cotton scouring efficiency = CBD융합단백질을 이용한 생화학적 표면분석기술

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Title
Thermostable β-glycosidase-CBD fusion protein for biochemical analysis of cotton scouring efficiency = CBD융합단백질을 이용한 생화학적 표면분석기술
Author(s)
Jae-Seok Ha; Y M Lee; Su Lim Choi; Jae Jun Song; C S Shin; J H Kim; Seung Goo Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 18, no. 3, pp. 443-448
Publication Year
2008
Abstract
Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline cellulose (Avicel), the reporter protein is guided to the cellulose fibers that are increasingly exposed as the scouring process proceeds. Among the tested reporter proteins, a thermostable β-glycosidase (BglA) from Thermus caldophilus was found to be most appropriate, showing a higher applicability and stability than GFP, DsRed2, or a tetrameric β-glucuronidase (GUS) from Escherichia coli, which were precipitated more seriously during the expression and purification steps. When cotton fabrics with different scouring levels were treated with the BglA-CBD and incubated with X-Gal as the chromogenic substrate, an indigo color became visible within 2 h, and the color depth changed according to the conditions and extent of the scouring.
Keyword
β-glycosidasebiochemical analysiscellulose binding domaincotton scouringfusion proteinthermus caldophilus
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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