DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyun Woo Oh | - |
dc.contributor.author | Sun Yeon Heo | - |
dc.contributor.author | Do Young Kim | - |
dc.contributor.author | Doo Sang Park | - |
dc.contributor.author | Kyung Sook Bae | - |
dc.contributor.author | Ho Yong Park | - |
dc.date.accessioned | 2017-04-19T09:09:55Z | - |
dc.date.available | 2017-04-19T09:09:55Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0003-6072 | - |
dc.identifier.uri | 10.1007/s10482-007-9210-2 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8372 | - |
dc.description.abstract | An exo-symbiotic bacterium capable of hydrolyzing xylan was isolated from the gut of the mole cricket, Gryllotalpa orientalis, and identified as Cellulosimicrobium sp. HY-12. The xylanase (XylACspHY-12) of this organism bound tightly to both DEAE and mono Q resins, and its molecular mass (Mr) was about 39.0 kDa. The highest xylanase activity was observed at pH 6.0 and 60°C. The enzyme was greatly suppressed by Ca2+, Cu2+, Co2+, and Fe2+ ions but not by Mg2+ and Mn2+. Although XylACspHY-12 was capable of hydrolyzing various types of xylosic compounds, it could not decompose carboxymethyl cellulose or xylobiose. The xylACspHY-12 gene consisted of an 1,188 bp open reading frame that encoded a polypeptide of 395 amino acids with a deduced molecular mass of 42,925 Da. The domain structure of XylACspHY-12 was most similar to those of the glycoside hydrolase (GH) family 10 endoxylanases. However its sequence identity with any of the enzymes in this family was below 52%. The results of this study suggest that the XylACspHY-12 is a new cellulase-free endo-β-1,4-xylanase with some properties that are distinct from those of GH family 10. | - |
dc.publisher | Springer | - |
dc.title | Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12 | - |
dc.title.alternative | Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12 | - |
dc.type | Article | - |
dc.citation.title | Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 442 | - |
dc.citation.startPage | 437 | - |
dc.citation.volume | 93 | - |
dc.contributor.affiliatedAuthor | Hyun Woo Oh | - |
dc.contributor.affiliatedAuthor | Sun Yeon Heo | - |
dc.contributor.affiliatedAuthor | Do Young Kim | - |
dc.contributor.affiliatedAuthor | Doo Sang Park | - |
dc.contributor.affiliatedAuthor | Kyung Sook Bae | - |
dc.contributor.affiliatedAuthor | Ho Yong Park | - |
dc.contributor.alternativeName | 오현우 | - |
dc.contributor.alternativeName | 허선연 | - |
dc.contributor.alternativeName | 김도영 | - |
dc.contributor.alternativeName | 박두상 | - |
dc.contributor.alternativeName | 배경숙 | - |
dc.contributor.alternativeName | 박호용 | - |
dc.identifier.bibliographicCitation | Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 93, no. 4, pp. 437-442 | - |
dc.identifier.doi | 10.1007/s10482-007-9210-2 | - |
dc.subject.keyword | Cellulosimicrobium sp. HY-12 | - |
dc.subject.keyword | Exo-symbiotic bacterium | - |
dc.subject.keyword | Glycoside hydrolase family 10 | - |
dc.subject.keyword | Xylanase | - |
dc.subject.local | Cellulosimicrobium sp. HY-12 | - |
dc.subject.local | Exo-symbiotic bacterium | - |
dc.subject.local | Glycoside hydrolase family 10 | - |
dc.subject.local | Xylanase | - |
dc.subject.local | xylanase | - |
dc.description.journalClass | Y | - |
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