Molecular parameters for assessing marine biotoxicity: gene expressions of rockfish(Sebastes schlegeli) exposed to polycyclic aromatic hydrocarbons

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Title
Molecular parameters for assessing marine biotoxicity: gene expressions of rockfish(Sebastes schlegeli) exposed to polycyclic aromatic hydrocarbons
Author(s)
S Woo; S Yum; Hong-Seog Park; J H Jung; S Lee; S J Kim; T K Lee
Bibliographic Citation
Molecular & Cellular Toxicology, vol. 3, no. 4, pp. 267-272
Publication Year
2007
Abstract
Environmental and anthropogenic changes affect the health and stability of marine ecosystem. In this study we aimed to identify molecular biomarkers for ecotoxicological pollutants risk assessment in the rockfish (Sebastes schlegeli). We designed primers based on conserved sequences by multiple alignments of target genes from related species, and cloned the partial cDNAs of cytochrome P450 (CYP1A1), glutathione S-transferase (GST), metallothionein (MT), superoxide dismutase (SOD), ubiquitin (UB), vitellogenin (VTG) and β-actin by reverse transcription polymerase chain reaction (RT-PCR) from S. schlegeli. Northern blot results indicated that these six genes expressions were significantly induced by benzo[a]pyrene (BaP, 1 μM) and that the level of each of their transcripts increased in BaP-exposed rockfish in a time-dependent manner. This study suggests that transcriptional changes in these six genes may be used for monitoring environmental exposure to polycyclic aromatic hydrocarbons (PAHs).
Keyword
sebastes schlegelienvironmental stressbenzo[a]pyrenebiomarkersgene expression
ISSN
I000-0184
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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